2,4,5-trichloroaniline

Endpoint summary

Description of key information

Trichloroaniline was administered for 14 days orally to rats at doses of 60 to 1000 mg/kg in a limited study. The reduced study design of this repeated dose study has only an orientating character. 
No haematological and clinical chemistry data were obtained in this study. Organ weights were only determined of liver, spleen, kidneys, testes and ovaries. Histopathological examination was only performed in the spleen and the liver.
However, no mortality occurred during the 14 day dosing period. The only clinical sign observed was the rough fur of three females of the high dose group beginning during the second treament week.
At 1000 mg/kg bw food consumption was comparable to control animals, the water consumption increased. Changes in the spleen of both sexes were described. Spleens were swollen (based on the increased blood content) and extramedullary haematopoesis and haematosiderosis were observed. The liver of one female of the 1000 mg/kg bw dose group showed a slight Kupffer cell siderosis. Liver weight (relative and absolute) was increased.
At 250 mg/kg bw a decreased body weight gain was noted (i.e. females 8% and 9% in the 250 and 1000 mg/kg dose group, respectively. At males of these dosing groups the decrease of body weight w as 4%). Food consumption of the 250mg/kg dose group was comparable to control animals. Females of this dose group showed changes in the spleen (haematosiderosis).
At 60 mg/kg bw animals showed a body weight gain comparable to the control animals. At this dose group there were no other findings.
The NOAEL is determined as 60mg/kg bw (based on no effects seen in the spleen and liver).

Key value for chemical safety assessment

Repeated dose toxicity: via oral route - systemic effects

Endpoint conclusion

Dose descriptor:

NOAEL

60 mg/kg bw/day

Additional information

Trichloroaniline is a known cause of methemoglobinemia. Although hematology has not been tested in this study the findings in liver (hemosiderosis) and spleen indicate a secondary effect of an action on the blood with consequent increased turnover of red cells.

Repeated dose toxicity: via oral route - systemic effects (target organ) cardiovascular / hematological: spleen; cardiovascular / hematological: other; digestive: liver