2,4,5-trichloroaniline

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: study was carried out according to test guidline and under GLP-conditions; no toxicity control

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,4,5-trichloroaniline

EC Number:

211-254-9

EC Name:

2,4,5-trichloroaniline

Cas Number:

636-30-6

Molecular formula:

C6H4Cl3N

IUPAC Name:

2,4,5-trichloroaniline

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Origin: Municipal sewage treatment plant Frankfurt- Sindlingen, Germany
Source: filtrated outlet of a municipal sewage treatment plant Frankfurt - Sindlingen, Germany
Pretreatment: aeration for 7 days
Used quantity: 0.5 ml/L
Preconditions: not pre-adapted to the test chemical and the reference chemical

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

4.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

A stock solution was prepared, adjusted to pH 7.7 by diluted NaOH solution and agitated for 28 hours. Final pH was 7.2. The tested sample being a saturated solution containing undissolved sediment was filtrated using a paper filter

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The reference test using sodium benzoic acid sodium salt as reference substance provided a degradation of %Deg. = 83% after 28 days. The degradation of the reference substance after 10 days amounted to more than 60%. The %Degr. value is based on the ratio of measured CO2 consumption and the theoretical CO2 consumption.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test item is not readily biodegradable (under the test conditions)

Executive summary:

The ready biodegradability of the test item 2,4,5-trichloroaniline was determined with a non adapted activated sludge in the Closed Bottle Test for a period of 28 days according to OECD guideline 301 D.

Both test item replicates did not reach the 10 % level (beginning of biodegradation) within 28 days. After 28 days the biodegradation was 0 %.

The validity criteria of the guideline are fulfilled.

The test item must be regarded as

not readily biodegradable

after 28 days.