Registration Dossier
Registration Dossier
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EC number: 210-415-0 | CAS number: 615-20-3
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 2002
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Unsuitable test system
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxic effects of Photodegradation Products of N-Methylbenzothiazol-2-one on Photosynthesizing Organisms
- Author:
- Noskova M, Kralova K, Gaplovsky A
- Year:
- 2 002
- Bibliographic source:
- Chemia i inzynieria ekologiczna,T.9, Nr.12, 1541-1546.
Materials and methods
- Principles of method if other than guideline:
- The effects of N-Methylbenzothiazol-2-one and its photodegradation (irradiation) products formed in tetrachloromethane on the photosynthetic electron transport in spinach chloroplasts were determined
- GLP compliance:
- no
Test material
- Reference substance name:
- N-Methylbenzothiazol-2-one
- IUPAC Name:
- N-Methylbenzothiazol-2-one
- Reference substance name:
- MBTO
- IUPAC Name:
- MBTO
- Reference substance name:
- Reaction products of N-Methylbenzothiazol-2-one and Tetrachloromethane formed during UV irradiation of the mixture
- IUPAC Name:
- Reaction products of N-Methylbenzothiazol-2-one and Tetrachloromethane formed during UV irradiation of the mixture
- Details on test material:
- The following substances were used in the experiments:
Mixture of tetrachloromethane and N-Methylbenzothiazol-2-one was exposted to UV light for 0, 1, 2.5, 4, 5.5, 7.5 h. The resulting mixture of several photodegradation products of both substances was used in the tests. This mixture contained N-Methylbenzothiazol-2-one and its photodegradation products formed in tetrachloromethane including 2-Chlorobenzothiazole.
Pure 2-Chlorobenzothiazole was not tested
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Irradiation products were dissolved in DMSO (final concentration up to 4 % v/v)
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- N-Methylbenzothiazol-2-one and its irradiation products formed after irradiation at different time intervals in a solution of the test substance in tetrachloromethane
N-Cyclohexylbenzothiazol-2-sulfenamide and its irradiation products formed after irradiation at different time intervals in a solution of the test substance in tetrachloromethane
Test organisms
- Species:
- other: Spinacia oleracea
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- isolated chloroplasts of spinach leaves
Study design
- Test type:
- other: photosynthetic electron transport in isolated spinach chloroplasts
- Study type:
- laboratory study
- Substrate type:
- other: in buffer solution
- Limit test:
- no
- Post exposure observation period:
- short time photosynthetic electron transport measurement ot determine toxic effects
Test conditions
- pH:
- 7.2
- Moisture:
- measurement of photosynthetic electron transport in dispersed isolated spinach chloroplasts of N-Methylbenzothiazol-2-one dissolved and irradiated in tetrachloromethane, and of the resulting products
- Details on test conditions:
- measurement of photosynthetic electron transport of substance dissolved and irradiated in tetrachloromethane. These artificial conditions are the onyl reason of 2-chlorobenzothiazole formation. In non-artifical systems, the likelyhood of 2-chlorobenzothiazole formation is extremely low due to missing of the reactive chlorine donor tetrachloromethane
- Nominal and measured concentrations:
- nominal (for substance mixture)
30 mg Chlorophyll/L in cloroplast preparations at test start
Start concentration of N-Methylbenzothiazol-2-one:
0, 1.5, 7.5, 15, 75, 150 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 2,6-Dichlorophenolindophenol
Results and discussion
Effect concentrationsopen allclose all
- Species:
- other: Spinacia oleracea chloroplast preparations
- Duration:
- 15 min
- Dose descriptor:
- other: IC50
- Effect conc.:
- 0.811 - 1.063 other: g/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Remarks:
- N-Methylbenzothiazole, untreated (non-irradiated, for comparison)
- Basis for effect:
- other: Photosynthetic electron transport
- Remarks on result:
- other: N-Methylbenzothiazole, untreated (non-irradiated, for comparison)
- Species:
- other: Spinacia oleracea chloroplast preparations
- Duration:
- 15 min
- Dose descriptor:
- other: IC50
- Effect conc.:
- 19.7 - 44.9 other: mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Remarks:
- irradiation products mixture
- Basis for effect:
- other: Photosynthetic electron transport
- Remarks on result:
- other: Irradiation without ultrasound
- Species:
- other: Spinacia oleracea chloroplast preparations
- Duration:
- 15 min
- Dose descriptor:
- other: IC50
- Effect conc.:
- 20.4 - 41.3 other: mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Remarks:
- irradiation products mixture
- Basis for effect:
- other: Photosynthetic electron transport
- Remarks on result:
- other: Irradiation with ultrasound
- Details on results:
- Only due to the presence of CCl4 the formation of 2-chlorobenzothiazole from N-Methylbenzothiazol-2-one (MBTO) is possible. The test system is not suited to attribute effects to specific substances. The test system also not suited to represent conditions in natural systems e.g. in vivo or in the environment
Any other information on results incl. tables
N-Methylbenzothiazol-2-one exhibited a slight inhibitory effect on the photosynthetic electron transport in spinach chloroplasts (IC50 = 0.8108 - 1.0627 g/L for pure substance before irradiation). After irradiation, the inhibitory effect was more pronounced (see above). The effect cannot be attributed to a single substance.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Remarks:
- in regard to 2-chlorobenzothiazole
- Conclusions:
- Due to in vitro test conditions, during photolysis of a mixture of N-Methylbenzothiazol-2-one (MBTO) and tetrachloromethane several irradiation products were formed including inter alii 2-chlorobenzothiazole. As these tests conditions do not represent the natural system, the results have no meaning for the assessment of the environmental properties of 2-chlorobenzothiazole. Due to the design of the study, the observed inhibitory effects of the irradiated mixture cannot be attributed to individual compounds.
- Executive summary:
study invalid in regard to 2 -chlorobenzothiazole
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