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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable with guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Deviations:
no
Principles of method if other than guideline:
1984 version of the guideline
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chlorobenzothiazole
EC Number:
210-415-0
EC Name:
2-chlorobenzothiazole
Cas Number:
615-20-3
Molecular formula:
C7H4ClNS
IUPAC Name:
2-chloro-1,3-benzothiazole
Details on test material:
purity 99.4 % w/w

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The substance was not completely dissolved at the 1000 mg/L concentration

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
The concentration of the sewage sludge was 6 g/L dry matter

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Remarks on exposure duration:
30 min and 180 min according to guideline

Test conditions

Hardness:
not relevant
Test temperature:
approximately 25 °C
pH:
not reported
Dissolved oxygen:
not reported
Salinity:
not reported
Nominal and measured concentrations:
nominal cocentrations:
100, 180, 320, 560, 1000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrations
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
176 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
in the range of expectations, activated sludge active and sensitive; study valid

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of the substance on activated sludge of predominantly domestic origin was 176 mg/L in a test on oxygen consumption by activated sludge according to ISO 8192 of 1984