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EC number: 210-415-0 | CAS number: 615-20-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Principles of method if other than guideline:
- Method is in most parts equivalent to the OECD TG 210 Alga, Growth inhibition test.
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-chlorobenzothiazole
- EC Number:
- 210-415-0
- EC Name:
- 2-chlorobenzothiazole
- Cas Number:
- 615-20-3
- Molecular formula:
- C7H4ClNS
- IUPAC Name:
- 2-chloro-1,3-benzothiazole
- Details on test material:
- no purity specified
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of stock solution (50.3 mg/L)
- test substance was weighed into water
- 60 s at 8000 rpm with an ultra-turrax
- stirred for 24 h on a magnetic stirrer
- finally filtered
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Desmodesmus subspicatus reported as Scenedesmus subspicatus
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
Test conditions
- Test temperature:
- 21-25 °C
- pH:
- without adjustment of pH. The pH is measured at the beginning of the test (0 h) and at its end (72 h).
- Dissolved oxygen:
- as oxygen is formed during photosynthesis, no oxygen measured
- Salinity:
- freshwater
- Nominal and measured concentrations:
- Stability of the test substance was verified by HPLC at 0 and 72 h. Recovery rates of the test substance (% of nominal):
- 78.8 - 98 at 0 h,
- 66.3 - 90 at 72 h
- Nominal test concentrations: 0.05, 0.11, 0.24, 0.53, 1.17, 2.58, 5.67, 12.47 and 27.44 mg/L without adjustment of pH - Details on test conditions:
- - Algal inoculum about 10E+04 cells/ml initial cell density
- 300 ml Erlenmeyer flasks with stoppers as test vessels
- Lighting 60-120 µE m-2 s-1
- Experimental design: 9 test concentrations plus control, 3 replicates per concentration, 6 replicates per control, highest test concentration without algae
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 4.50-7.28 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.29 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 5.07 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 9.06-10.25 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.29 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 5.07 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Reported statistics and error estimates:
- The 72 h-EC50 values are calculated or read from the concentration/percentage response curve.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 of the substance is 9.64 mg/L (nominal) in a 72 h algae growth test according to Directive 92/69/EEC, C.3 with Desmodesmus subspicatus. The NOEC based on biomass growth (b) was identical to the based on population density growth (r) NOEC = 2.29 mg/L
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