3-methyl-1,3-butandiol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 May - 16 July 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

During the definitive test, the temperature of the test area was maintained at 20.9 ± 1.1°C. However,
the protocol states that the temperature of the test area should be constant to within ± 1.0°C.

Deviations:

yes

Remarks:

As the validity criteria for this test were met, this deviation from protocol does not affect the integrity of the study.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

During the definitive test, the temperature of the test area was maintained at 20.9 ± 1.1°C. However,
the protocol states that the temperature of the test area should be constant to within ± 1.0°C.

Deviations:

yes

Remarks:

As the validity criteria for this test were met, this deviation from protocol does not affect the integrity of the study.

GLP compliance:

yes

Specific details on test material used for the study:

Appearance: Clear, colourless liquid
Storage conditions: Room temperature in the dark
Batch number: 52834
Expiry date: December 2006
Purity: 99%

Analytical monitoring:

yes

Details on sampling:

The aqueous samples were diluted 1 ml to 20 ml with methanol to bring the expected analyte concentration within the calibration range. The diluted samples were analysed for the test item by gas liquid chromatography (GLC) using flame ionisation detection.

Vehicle:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: Elendt M4 meduim

Test organisms (species):

other aquatic crustacea: DM

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Feeding during test: yes
- Food type: suspension of the unicellular green alga
- Amount: 0.1 to 0.2 mg carbon per daphnid
- Frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

238 mg CaCO3/L

Test temperature:

20.9 ± 1.1°C

pH:

7.55 - 7.86

Dissolved oxygen:

99 - 105 % air saturation value

Nominal and measured concentrations:

nominal test concentration:100 mg/l
measured test concentration:101 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass dishes containing 100 ml of medium
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

OTHER TEST CONDITIONS
- Photoperiod:16 hours light : 8 hours dark was maintained, with periods of subdued lighting at the beginning and end of each light phase.

RANGE-FINDING STUDY
- Test concentrations: nominal test concentrations of 1, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: Based on the results of this range finding test, the definitive test employed a single concentration, nominally 100 mg/l

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 101 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

not determinable

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 101 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

not determinable

Details on results:

After 48 hours, no significant immobility (<10%) had occurred.
The measurements of water quality (temperature, pH, dissolved oxygen, total hardness and alkalinity) remained within acceptable limits during the study.

Results with reference substance (positive control):

The sensitivity of juvenile Daphnia cultured in this laboratory is periodically assessed using the reference substance potassium dichromate. The results for the most recent test performed prior to this study indicated that its 48-hour EC50 to Daphnia magna was 0.7 mg/l; this was within the range typically obtained in this laboratory (0.3 to 0.8 mg/l).

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 of MBD for the immobilisation of Daphnia magna could not be identified but must be >101 mg/l.
The ‘no-observed effect concentration’ of MBD with Daphnia magna was >101 mg/l.

Description of key information

48-hour EC50 value: >101 mg/l

“No observed effect concentration” : >101 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

101 mg/L