3-methyl-1,3-butandiol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

7 June - 26 July 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Appearance: Clear, colourless liquid
Storage conditions: Room temperature in the dark
Batch number: 52834
Expiry date: December 2006
Purity: 99%

Analytical monitoring:

yes

Details on sampling:

On each occasion, the test substance was dissolved in a volumetric flask containing diluent water. The contents of the flask were shaken before being poured into a test vessel (glass aquarium) containing diluent water and the volume adjusted to 25 litres with water.
Four, mid-vessel samples (100 ml) of medium were taken from the control and test vessels at 0 and 72 hours (fresh medium) and at 24 and 96 hours (expired medium) for analysis. On each occasion, the samples were stored in a refrigerator for up to two days before they were analysed; the others were stored in a refrigerator in case further analysis was required.

Vehicle:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: Dechlorinated tap water

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Age at study initiation (mean and range, SD): eggs hatched in March 2005
- Length at study initiation (length definition, mean, range and SD): 5.43 cm
- Weight at study initiation (mean and range, SD): 2.38 g
- Maintenance of the brood fish: aerated supply of diluent water under flow-through conditions

ACCLIMATION
- Acclimation period: 14 days
- Type and amount of food during acclimation: commercial fish food, 2.5% of the total wet-weight of fish
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): <1%

No food was given during the 21-hour period immediately before exposure or during the exposure period itself. No medication was given during the holding period

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

CA 180 mg CaCO3/L

Test temperature:

15.5 ± 0.5°C

pH:

7.56 - 8.31

Dissolved oxygen:

81 - 99 % air saturation value

Conductivity:

350 µS/cm (20°C)

Nominal and measured concentrations:

nominal concentration: 100 mg/l
measured concentration: 103 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 25 litres of medium to a depth of 17 cm
- Aeration: yes
- No. of organisms per vessel: 1
- Biomass loading rate: initial static loading of 0.95 g bodyweight/litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered dechlorinated tap water blended with tap water that had been softened

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light: 8 hours dark with periods of subdued lighting at the beginning and end of each light phase

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : death, absence of respiratory movement, absence of response to physical stimulation of the caudal peduncle

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1, 10 and 100 mg/l

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 103 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 103 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The measurements of water quality (temperature, pH, concentrations of dissolved oxygen and total hardness) remained within acceptable limits throughout the study.

Validity criteria fulfilled:

yes

Conclusions:

No mortality or treatment-related effects on the fish were exhibited during the study.
Based on these findings the following values have been estimated:
96-hour LC50 value : > 103 mg/l.
“No observed effect concentration” : > 103 mg/l.

Description of key information

96-hour LC50 value : > 103 mg/l.

“No observed effect concentration” : > 103 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

103 mg/L