3-methyl-1,3-butandiol

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 July 1993 - 21 September 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

Principles of method if other than guideline:

"Method for testing the biodegradability of chemical substances by microorganisms" in the "Testing Methods for New Chemical Substances" (July 1974, No. 5, Planning and coordination Bureau, Environment Agency; No. 615, Pharmaceutical Affairs Bureau, Ministry of Health and Welfare; and No. 392, Basic Industries Bureau, Ministry of International Trade and Industry, Japan), and "Ready Biodegradability: Modified MITI Test (I) (OECD Guideline No. 301C, revised July 1992)".

GLP compliance:

yes

Specific details on test material used for the study:

Purity: 99 %
Storage condition: Cold storage place
Stability: The test substance was stable under the storage conditions

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): on-site sampling was carried out at 10 locations
- Method of cultivation: old and fresh activated sludge were mixed and aerated. Synthetic sewage was added to the mixture

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium:
- Additional substrate: synthetic sewage
- Test temperature: 25 ± 1°C
- pH: 7.0 ± 1.0
- pH adjusted: yes
- Aeration of dilution water: yes
- Suspended solids concentration:
- Continuous darkness: yes/no
- Other:

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment: close system oxygen consumption measuring apparatus
- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:

STATISTICAL METHODS:

Reference substance:

aniline

Key result

Parameter:

% degradation (O2 consumption)

Value:

100

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance):
62.5 % degradation after 7 d
77 % degradation after 14 d
88.5 % degradation after 21 d
94.5 % degradation after 28 d

Results with reference substance:

Points of degradation plot (reference substance):
41 % degradation after 7 d
81 % degradation after 14 d
83 % degradation after 21 d
84 % degradation after 28 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

It is concluded that the test substance is biodegraded by microorganisms under the present test conditions.

Description of key information

Percentage of biodegradation (average %)

BOD: 94%

TOC: 98%

GC: 100%

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable