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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
30 July 1993 - 21 September 1993
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
not specified
Principles of method if other than guideline:
"Method for testing the biodegradability of chemical substances by microorganisms" in the "Testing Methods for New Chemical Substances" (July 1974, No. 5, Planning and coordination Bureau, Environment Agency; No. 615, Pharmaceutical Affairs Bureau, Ministry of Health and Welfare; and No. 392, Basic Industries Bureau, Ministry of International Trade and Industry, Japan), and "Ready Biodegradability: Modified MITI Test (I) (OECD Guideline No. 301C, revised July 1992)".
GLP compliance:
Specific details on test material used for the study:
Purity: 99 %
Storage condition: Cold storage place
Stability: The test substance was stable under the storage conditions
Oxygen conditions:
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): on-site sampling was carried out at 10 locations
- Method of cultivation: old and fresh activated sludge were mixed and aerated. Synthetic sewage was added to the mixture
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
- Composition of medium:
- Additional substrate: synthetic sewage
- Test temperature: 25 ± 1°C
- pH: 7.0 ± 1.0
- pH adjusted: yes
- Aeration of dilution water: yes
- Suspended solids concentration:
- Continuous darkness: yes/no
- Other:

- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment: close system oxygen consumption measuring apparatus
- Details of trap for CO2 and volatile organics if used:
- Other:

- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:

Reference substance:
Key result
% degradation (O2 consumption)
Sampling time:
28 d
Details on results:
Points of degradation plot (test substance):
62.5 % degradation after 7 d
77 % degradation after 14 d
88.5 % degradation after 21 d
94.5 % degradation after 28 d
Results with reference substance:
Points of degradation plot (reference substance):
41 % degradation after 7 d
81 % degradation after 14 d
83 % degradation after 21 d
84 % degradation after 28 d
Validity criteria fulfilled:
Interpretation of results:
readily biodegradable
It is concluded that the test substance is biodegraded by microorganisms under the present test conditions.

Description of key information

Percentage of biodegradation (average %)

BOD: 94%

TOC: 98%

GC: 100%

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information