Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 1- June 1983

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Ames test 5 Salmonella strains and triplicate plates

Version / remarks:

oecd short term tox group - no Ecoli strain

Deviations:

no

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

EC Number:

283-381-8

EC Name:

Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

Cas Number:

84604-96-6

Molecular formula:

C38H76N2S4Zn

IUPAC Name:

bis({[bis(3,5,5-trimethylhexyl)carbamothioyl]sulfanyl})zinc

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Purity 98%
BN 2152
stored at room temperature

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

with S-9 mix ( 2 aminoanthracene for strains TA1535, TA1537, TA1538, TA98 and TA100

Test concentrations with justification for top dose:

On each bacterial strain, four concentrations of test substance was assessed. he highest concentration will usually be 0.05g of test substnce dissolved in 1.0ml of solvent. Three 10 fld serial dilutions of the top concentrations will also be tested.

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

yes

Positive controls:

yes

Positive control substance:

9-aminoacridine

sodium azide

other: 2 aminoanthracene

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

Zinc Diisononyldithiocarbamate was non toxic towards the tester strains. Therefore the 5000 microgramme/plate was chosen as the top dose level in mutation tests.
The mean number of revertant colonies, together with the individual plate counts for Zinc Diisononyldithiocarbamate obtained in the first mutation test with tester strains TA 1535, TA1538, TA98 anmd TA100 are shown in tbale 2 and the sterility and positive controls in table 3.

No substantial increases int he revertant colony numbers of any of the five strains were observed following treatment with Zinc Diisononyldithiocarbamate at any dose level, either in presence or absence of liver microsomal fraction (S9 mix)

Applicant's summary and conclusion

Conclusions:

No evidence of mutagneic potential of test item was obtained in this bacterial test system.