Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

aug 2012- April 2013

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

EC Number:

283-381-8

EC Name:

Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

Cas Number:

84604-96-6

Molecular formula:

C38H76N2S4Zn

IUPAC Name:

bis({[bis(3,5,5-trimethylhexyl)carbamothioyl]sulfanyl})zinc

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Purity > 97 %
BN 1120039.2
expiry July 2013
storage - room temp in the dark

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Study used 1st instar Daphnia magna derivd from in house lab cultures at Harlan. Daphnia were maintained in 150ml glass beakers containing Elendt M7 medium in temperature controlled room at approx 20°C. The lighting cycle was controlled to give 16 hours light and 8 hours darkness cycle. Each culture was fed daily with mixture of algal suspension, culture conditions ensured the reproduction was by parthenogenesis. Gravid adults were isolaed the day before initiation of test, such the young daphnids were less than 24 hours old.

Study design

Test type:

static

Water media type:

other: reconstituted water

Limit test:

yes

Total exposure duration:

48 h

Test conditions

pH:

8.1

Details on test conditions:

a modificationof the standard method for sample preparation was performed, in view of difficulties with evaluation of aquatic toxicity of poorly soluble test items, using OECD 2000 guidelines for difficult substances. Using htis approach, a saturated solution was prepared by stirring an excess (100mg/L) of test item in reconsistuted water for a preiod of 24 hours prior to removing any undissolved test item by filtration.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 - ca. 100 other: % v/v saturated solution

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Any other information on results incl. tables

No immobolisation was observed at the test concentration of 0.1, 1.0, 10 and 100% v/v saturated solution in range finder. The definitive test was carried out with 100% v/v and no immobisation was recorded. NOEC after 24 and 48 hours exposure was 100% v/v saturated solution

results from positive control were within normal range for this reference item - see table below. NOEC conc after 24 and 48 hours were 0.56 and 0.32 mg/l resp.

Time (h)	EC 50 mg/l)	95% confidence limit (mg/l)
24	0.75	0.56 -1.0
48	0.45	0.42- 0.48

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Acute toxicity of test item to Daphnia magna was investigated, no toxicty was recorded at all concentrations and study gave a 48 hour EC50 > 100% v/v saturated solution. THe NOEC was 100% v/v saturated solution