Registration Dossier
Registration Dossier
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EC number: 283-381-8 | CAS number: 84604-96-6
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- mar 1995- June 1995
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EPA 540/9-86- 138 to meet requirements of EPA TSCA (40 CFR.797.1600)
- Version / remarks:
- 1986
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc
- EC Number:
- 283-381-8
- EC Name:
- Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc
- Cas Number:
- 84604-96-6
- Molecular formula:
- C38H76N2S4Zn
- IUPAC Name:
- bis({[bis(3,5,5-trimethylhexyl)carbamothioyl]sulfanyl})zinc
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Supplied by Robinsons
Batch no 93102
White powder,
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Strain: Pimephales promelas (brrod stock bn 94/122, embryos 95/27 less than 24 hours old
- Source: Mulitaquaculture systems in NY USA
- Age at study initiation (mean and range, SD): less than 24 hours old, eggs eamined microscopically at initiation of study
- Length at study initiation (length definition, mean, range and SD): 32 days post hatch ( 36 days in total)
- Weight at study initiation (mean and range, SD):
- Method of breeding:
- Feeding during test
- Food type: high protein fish food
- Amount:
- Frequency:
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency during acclimation:
- Health during acclimation (any mortality observed):
QUARANTINE (wild caught)
- Duration:
- Health/mortality:
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 36 d
Test conditions
- Hardness:
- mean 43.7 mg/l as CaCO3
- Test temperature:
- 24.7- 24.9 mean
- pH:
- ranged from 7.45 to 8.01 pH
- Dissolved oxygen:
- 7.5-7.9 mg/l mean
- Conductivity:
- mean 216 microseconds per cm
- Nominal and measured concentrations:
- nominal 0.36mg/l as this was the limit of solubility of test item
measured concentrations on multiple days, mean 0.3mg/l . The mean measured concentration as percentage of nominal conc as pooled replicates 83% - Details on test conditions:
- A dynamic flow through test system was used to ensure a constant exposure of test item
- Reference substance (positive control):
- no
- Remarks:
- solvent control 100µl/l of dimtheyl formamide
Results and discussion
Effect concentrations
- Key result
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.36 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Remarks:
- mean measured 0.3mg/l
Any other information on results incl. tables
hatchability of minnow embryos was not significantly affected (P=0.05) The percentage hatchability in individual replicates including controls ranged from 85 -100% with an overall mean of 96.7% . The LOEC for this parameter was, therefore greater than the concentration tested 0.36 mg/l (nominal)
Larval survival was not signifacntly affected (p=0.05) The larval survival for all concentrations ( individual replicates) ranged from 94.1 to 100% with an overall mean of 96.5% based on total number of larvae released by exposure day 5. The LOEC for this parameter was therefore greater than the concentration tested 0.36mg/l
Larval growth in comparison with dilution water controls (pooled) no significant reduction (p=0.05) found in lengths or weights of the larvae at 32 days post hatch
Further tables attached
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The overall NOEC of test item was > 0.36 mg/l ( nominal concentration and at limit of solubility) - however no toxic effects were observed throughout study
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