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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Aug -Sep 1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Robinsons ref 2152
- Expiration date of the lot/batch: not stated in study
- Purity test date: 98% purity - date of receipt May 1983

Storage : in original container at room temperature

Details on test solutions:
Ultrasonic dispersion and progressive dilution over a 20 min period using 0.1g Tween 80/g as a wetting agent ( no other solvent was used since precipitation and flocculation occurred immediately on mising with water)
Test organisms (species):
Salmo sp.
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Strain:Salmo gairdneri
- Source: Parkwood trout farm, kent
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 5.2cm
- Weight at study initiation (mean and range, SD): mean weight 3.1g
- Method of breeding:
- Maintenance of the brood fish:

ACCLIMATION
- Acclimation period: 16- 30 aug 1983
- Acclimation conditions (same as test or not):
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: Omega trout pellets daily. Discontinued 24 hours prior to test
- Health during acclimation (any mortality observed):
Test type:
static
Water media type:
other: dechlorinated lab tap water
Limit test:
yes
Total exposure duration:
96 h
Hardness:
total harness 292 mg/l
Test temperature:
14 +/- 1
pH:
7.6
Dissolved oxygen:
aeration via narrow bore glass tubes
Nominal and measured concentrations:
nominal 10mg/l
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
>= 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
mortality (fish)
Details on results:
5% mortality after 96hrs exposure using 20 fish.
Sublethal observations / clinical signs:

 

                cumulative mortality, 3h, 6h, 24h, 48h, 72h, 96h

 control (10 fish

 0

 0

 group 1 (10 fish)

 0

 0

 0

 0  1  1
 group 2 (10 fish)  0
Validity criteria fulfilled:
yes
Conclusions:
No actute aquatic toxicity observed.

EC50> 10mg/l (nominal )

Description of key information

oecd 203 acute fish study

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
10 mg/L

Additional information

No toxicity observed at nominal concentration 10mg/l

Lc 50 > 10mg/l (nominal)