Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

range finding initially then definitive report May 1995 to June 1995

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

EC Number:

283-381-8

EC Name:

Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc

Cas Number:

84604-96-6

Molecular formula:

C38H76N2S4Zn

IUPAC Name:

bis({[bis(3,5,5-trimethylhexyl)carbamothioyl]sulfanyl})zinc

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Specific details on test material used for the study:

White powder
BN 2018973102
date received July 1994
storage conditions - room temperature

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

test carried out using 1st instar Daphnia magna ( staus) maintained in a laboratory culture originating from a strain suplied by the institut National de Recherche chemique appliquee (IRCHA) France.
test species were maintained in 2L dechlorinated tap water at a temp of 21°C. THe lighting cycle was controlled to give 16 hours light and 8 hours darkness. Each culture fed daily with mixed algae ( Chlorella Spp) Gravid adults were isolated 24 hours prior to initiation of the test, then young Daphnid produced overnight were then removed for testing.

Study design

Test type:

semi-static

Water media type:

other: dechlorinated tap water

Limit test:

yes

Total exposure duration:

21 d

Remarks on exposure duration:

semi static test item renewal

Post exposure observation period:

only 21 days

Test conditions

Hardness:

lab tap water dechlorinated by passage through an activated carbon filter was used with a total harndess of approximately 116mg/l as CaCo3

Test temperature:

21 °C @ 1°C

pH:

7.8

Dissolved oxygen:

21

Nominal and measured concentrations:

Verification of test concentrations showed the measured test concentration to range between 11 - 148% of nominal - variable results obtained are considered to be due to the analytical variation using the headspace gas chromatography method of analysis.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 0.32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

immobilisation

Effect concentrations 2

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 0.32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

see attached on page 15

Conclusions:

NOEC immobilisation > 0.32mg/l (nominal)
NOEC reproduction > 0.32 mg/l ( nominal )