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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The current study was conducted according the Guideline OECD 301D under GLP. This study is not the most suitable study due to the low water solubility
Justification for type of information:
The performed OECD 301D (closed bottle) is not the most suitable method for this substance as the water solubility is very low. The concentrations used in the study were above the water solubility of the substance. During the study no agitation was used to keep the chemical dispersed, which could lead a lower bioavailability for the bacteria in the inoculum. Based on the similarity of the structure of both substance it would be expected that liquid AKD would also be readily biodegradable. In particular, the double bond in the chain could lead to a faster break down of the structure.
A new readily biodegradation study (OECD 301B) will be performed to confirm the readily biodegradability of the substance.

Data source

Reference Type:
study report

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(4E)-4-(C13-C17)alkenylidene-3-(C12-C16)alkenyl oxetan-2-one
EC Number:
Cas Number:
Molecular formula:
Cx Hy O2 with x ranges 32 to 36 and y ranges from 56 to 66 Molecular formula of the main dimer: C36H64O2 ( dimer from 2 C18:1 fatty acids)
(4E)-4-(C13-C17)alkenylidene-3-(C12-C16)alkenyl oxetan-2-one

Study design

Inoculum or test system:
other: secondary effluent of a municipal treatment plant
Duration of test (contact time):
28 d
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradation
% degradation (CO2 evolution)
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Reference substance demonstrated 63% degradation at day 7, 76% at day 14, 75% at day 21, and 80% at day 28. This was considered valid.

Applicant's summary and conclusion