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Toxicity to terrestrial plants

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Description of key information

A seedling emergence and seedling growth test in 3 test species with the source substance showed that the 28-d EC50 for growth, as well as the 14-d EC50 for germination, is >1000 mg/kg dw for all species. The lowest NOEC was determined to be 100 mg/kg dw (for 2 of the 3 species).

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Executive summary of the study performed with the source substance:

The OECD 208 terrestrial toxicity test on plants was performed with AQUAPEL 364 for 28 days. Test concentrations were 1.0, 10, 100, 1000 mg/kg dry soil, nominal. The test species were Oat (Avena sativa), Sunflower (Helianthus annuus) and Mung bean (phaseolus aureus). 4 replicate pots were prepared for controls and test concentrations each containing 9 seeds, later thinned to maximally 5. Soil (50% sterilised loam and 50% gravel) was 9:1 mixed with silver sand previously coated with test substance by means of a vehicle (chloroform). The solvent was evaporated prior to the start of the study. Both germination (up to day 14) and growth (day 28) were studied as well as a visual assessment of plant condition was made on day 14 and 28. In the highest dose groups (1000 mg/kg dry soil) the top soil was dry, likely due to the water repellent properties of the test substance. This likely caused - only at this dose - a reduced germination. The results show that both the EC50 (14days) for germination as well as the EC50 (28 days) for growth for all species is > 1000 mg/kg dry soil based on nominal concentration.