α,α,α,2-tetrachlorotoluene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α,2-tetrachlorotoluene

EC Number:

218-377-7

EC Name:

α,α,α,2-tetrachlorotoluene

Cas Number:

2136-89-2

Molecular formula:

C7H4Cl4

IUPAC Name:

1-chloro-2-(trichloromethyl)benzene

Details on test material:

Purity: 97.0 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test item was mixed with water at 30°C and homogenised using an Ultra-Turrax and by ultrasonic treatment.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Strain: Hamilton-Buchanan
- Source: West Aquarium Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): 3 months
- Length at study initiation (mean, range and SD): 2.8 cm, 2.4 - 3.4 cm, 0.20 cm
- Method of breeding: 22 +- 1 °C; O2-content >= 80% ; 12 h day/night cycle, 700 Lux; <= 1g fish/l water;
- Feeding during test: no feeding during test

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Type and amount of food:
- Food type: Tetra Min, Tetra Werke, Melle, Germany;
- Amount: ad libitum;
- Frequency: 2 times a day;

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Test temperature:

Test groups: 21.0 - 22.8 °C
Control groups: 21.6 - 22.7 °C

pH:

Test groups: 6.4 - 8.0
Control groups: 7.3 - 8.1

Dissolved oxygen:

Test groups: 6.2 - 8.8 mg/l
Control groups: 7.0 - 9.3 mg/l

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

350 mg/L

Nominal / measured:

nominal

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

350 mg/L

Nominal / measured:

nominal

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 500 - <= 710 mg/L

Nominal / measured:

nominal

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 500 - <= 710 mg/L

Nominal / measured:

nominal

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

710 mg/L

Nominal / measured:

nominal

Details on results:

A probit analysis to determine the LC50 was not possible given the distribution of the effective concentrations and the dilution factors used.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

From this study, LC0 (48 h and 96 h) for 2-chlorobenzotrichloride was determined to be 350 mg/L, LC50 (48 h and 96 h) to be 500 - 710 mg/L and LC100 (496 h) to be 710 mg/L.