α,α,α,2-tetrachlorotoluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23-06-1981 to 24-06-1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38 412 Part 11

Version / remarks:

related to OECD Guidelline 202, Guideline 84/449/EWG (C2) and ISO 6341 but the incubaiton time is different (24 hrs vs 48hrs)

Deviations:

not specified

GLP compliance:

no

Remarks:

GLP not in force at the time of the study

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α,4-tetrachlorotoluene

EC Number:

226-009-1

EC Name:

α,α,α,4-tetrachlorotoluene

Cas Number:

5216-25-1

Molecular formula:

C7H4Cl4

IUPAC Name:

1-chloro-4-(trichloromethyl)benzene

Test material form:

solid

Specific details on test material used for the study:

A saturated solution preapred under hydrolytic conditions for the test-substance: A suspension of the substance in de-ionised water was adjusted to pH 12 with NaOH, stirred for 72 hours at 25C and filtered.
Analysis of stock solution: 304 mg DOC/L
concnertation of stock solution

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

Analysis of stock solution: 304 mg DOC/L
concentration of stock solution 831.4 mg/L calculated fro DOC of the solution and carbon contnet ofthe substance of 36.57%

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: straus
- Source: own laboratory strain
- Age of parental stock (mean and range, SD): not given
- Feeding during test -not given
- Food type: algae (Scenedesmus supspicatus)
- Amount:
- Frequency: twice daily

Breeding: cultivated adult animals in glass vessels (4L) canging the medium once a week and feeding the animals twice a day. Medium : Synthetic water according to DIN 38 412 part 11

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Hardness:

as per DIN 38 412 part 11

Test temperature:

21 C thermostatically controlled

pH:

as per DIN 38 412 part 11

Dissolved oxygen:

not determined

Salinity:

as per DIN 38 412 part 11

Conductivity:

as per DIN 38 412 part 11

Nominal and measured concentrations:

Nominal concentrations: 8.31, 83.1, 103.9, 166.3, 415.7, 831.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100ml glass vessel
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 100mL
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A
- Renewal rate of test solution (frequency/flow rate): N/A
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: 10 animals per vessel

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water according to DIN 38 412 part 11
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: conducted in dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : motility

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

potassium dichromate 2mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

ca. 112 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 210 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

other: EC90

Effect conc.:

ca. 394 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

70% mean immobilised daphnia following 24hr exposure to 2mg/L potassium dichromate

Reported statistics and error estimates:

EC values calculated by logarithmic regression of the measured data
EC10 95% CI = 42-131 mg/L standard error 104-118 mg/L
EC50 95% CI = 188-417 mg/L standard error = 202-221 mg/L
EC90 95% CI= 271-4203 mg/L standard error = 346-472 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Considering the incubation time of only 24 hours it cannot be excluded that a test according to OECD202 would result in a range of 10-100 mg/L for the EC50 value of the hydrolysed solution of the test substance to Daphnia magna