2-chlorophenyl isocyanate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

other: acute oral toxicity study in rats

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorophenyl isocyanate

EC Number:

222-023-7

EC Name:

2-chlorophenyl isocyanate

Cas Number:

3320-83-0

Molecular formula:

C7H4ClNO

IUPAC Name:

1-chloro-2-isocyanatobenzene

Details on test material:

Contents: 99.7%,
Molecular weight: 153.5 g/mole

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Doses:

200, 2000 mg/kg bw.

No. of animals per sex per dose:

5

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Any other information on results incl. tables

Results:

 

Dose ( mg/kg b.w.)	Toxicological results*			Duration of signs	Time of death	Mortality (%)
Male
200	0	0	5			0
2000	0	5	5	2h - 7h		0
Female
200	0	0	5			0
2000	2	5	5	1h - 11d	3d - 11d	40

*1st figure= number of dead animals

2nd figure= number of animals with signs

3rd figure=number of animals in the group

Applicant's summary and conclusion

Executive summary:

A study of acute oral toxicity in male and female Wistar rats was conducted with the test substance.

Following doses were tested by gavage: 200, 2000 mg/kg bw.

A dose of 200 mg/kg body weight was tolerated by male and female rats without signs and without mortalities. Clinical signs observed at 2000 mg/kg body weight consisted of piloerection, decreased motility and reactivity,uncoordinated and spastic gait, tachypnea, labored breathing, breathing sounds, diarrhea, and narrowed palpebral fissure, occurred within 1 hour and 2 days after administration, and usually lasted until 7 hours to 3 days. Body weight and body weight gain were not affected by treatment.

General autolysis was observed in animals that died during the observation period. The gross pathology investigations performed at the end of the follow-up period did not afford any findings indicative of a specific test compound effect.

The LD 50 was found to be >2000 mg/kg bw.