2-chlorophenyl isocyanate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline Study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Control studies are performed under GLP-conditions but without assignment to a particular study. This allows use of control data for several studies that have been performed under similar experimental conditions within a recent time frame. This procedure is in compliance with current testing guidelines as well as animal welfare regulations.

GLP compliance:

yes

Test type:

other: acute inhalation toxicity test in rats

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorophenyl isocyanate

EC Number:

222-023-7

EC Name:

2-chlorophenyl isocyanate

Cas Number:

3320-83-0

Molecular formula:

C7H4ClNO

IUPAC Name:

1-chloro-2-isocyanatobenzene

Details on test material:

Purity: 99.7 %,
Molecular weight: 153.5 g/mol

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

nose only

Vehicle:

clean air

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Remarks on duration:

nose-only conditions

Concentrations:

32, 47, 63, 125, and 184 mg/m³ air.

No. of animals per sex per dose:

5

Control animals:

yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

ca. 47 mg/m³ air

Exp. duration:

4 h

Any other information on results incl. tables

Results:

LC50 inhalation (vapour, 4 hr	NO(A)EL
Males and females combined ca. 47 mg/m³ air*	Males and females: <32 mg/m³ air*

* All concentration data represent actual concentrations of the test substance in the rats breathing zone.

Observations and measurements:

Exposure to vapour concentration of 32 mg/m³ did not induce test substance related mortality. Exposure to 47 mg/m³ air and above was followed by a characteristic type of mortality pattern occurring within post exposure days 1 through 18. Marked gender specific differences in susceptibility was not observed. Exposures to concentrations of 32 mg/m³ and above were followed by a concentration-dependent signs indicative of or secondary to respiratory tract irritation, such as bradypnea, dyspnea, labored breathing pattern, rales, nostrils with red encrustrations, cyanosis, prostration (lying on side and belly), reduced motility, ungroomed hair coat, hypothermia, decreased body weights and piloerection. The duration of signs (maximum duration up to day 28), in most instances, was dependent on respiratory signs. Gross necropsy (among others) revealed less collapsed, consolidated, discolorated (dark-red) lungs with serous mucus in the airways, including trachea. The lung associated lymph nodes were enlarged. Clinical observations and necropsy findings support the conclusion that a causal relationship between lethality and lung damage existed.

Applicant's summary and conclusion

Executive summary:

A study on the acute inhalation toxicity of the test substance in male and female Wistar rats has been conducted in accordance with OECD Guideline No. 403. Groups of rats were nose-only exposed for four hours to average vapour concentrations of 32, 47, 63, 125, and 184 mg/m³ air.

The LC50 for males and females combined was found to be ca. 47 mg/m³ air.