Hydrogen [4-[[4-(diethylamino)phenyl][4-[ethyl[(3-sulphonatobenzyl)amino]-o-tolyl]methylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(3-sulphonatobenzyl)ammonium, sodium salt

Density

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method A.3 (Relative Density)

GLP compliance:

yes (incl. QA statement)

Type of method:

pycnometer method

Type:

relative density

Density:

1.273 other: dimensionless

Temp.:

20 °C

Literature data (CRC Handbook of Chemistry and Physics 75th Edition)

d (20, w) (density of water at 20 °C) = 0.9982 g/mL

d (4, w) (density of water at 4 °C) = 0.99997 g/mL

d (20, a) (density of air at 20 °C) = 0.0012 g/mL

Conclusions:

The relative density of Special Brilliant Blue FFR at 20 °C was determined as 1.273.

Executive summary:

The relative density of Special Brilliant Blue FFR was determined according to OECD 109, which is in all relevant parts similar to EC A.3. The solid sample was accurately weighed into a 20 mL pycnometer and by displacement of the solvent (n-heptane), its equivalent volume was determined. Finally, the density of the sample was calculated.

The relative density of Special Brilliant Blue FFR at 20 °C was determined as 1.273.

Reference 2

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7300 (Density / Relative Density / Bulk Density)

Qualifier:

according to guideline

Guideline:

other: CIPAC MT 3 Specific Gravity, Density, and Weight per Millilitre

GLP compliance:

yes (incl. QA statement)

Other quality assurance:

other: SOP-LA-031

Remarks:

Relative Density (Solids) with gas comparison pycnometer

Type of method:

air comparison pycnometer (for solids)

Type:

relative density

Density:

1.3 other: dimensionless

Temp.:

20 °C

Two tests with different amounts of the test item were performed to determine the relative density D₄²⁰ at a temperature of 20 °C ± 0.01 °C. Table 5 compiles the results.

Table 5:       Results of the measurement of the density of the test item

Test No.	Temperature [°C]	Amount of test item [g]	Average sample volume [cm³]	Density [g/cm³]*
1	20	9.36	7.22822	1.29525
2	20	8.67	6.67461	1.29920

* The pressurizing-expansion cycles are performed until 5 subsequent volume measurements have a relative standard deviation (RSD) of ≤ 0.02 %.

The mean density of the test item at 20 °C is 1.297225 g/cm³. The relative density D₄²⁰ is calculated by dividing the density of the test item by the density of water at 4 °C (1.297257 g/cm³).

The relative density is D₄²⁰ = 1.30.

Conclusions:

The relative density of the test item is 1.30 at 20°C.

Executive summary:

According to OECD Test Guidelines 109 (2012), Regulation EC No. 440/2008 Method A.3. and EPA OCSPP Test Guideline 830.7300 (2002), the relative density D420 of the test item Hydrogen [4-[[4-diethylamino)phenyl][4-[ethyl[(3-sulphonatobenzyl)amino]-o-tolyl]methylene]-3-methylcylohexa-2,5-dien-1-ylidene](ethyl)3-sulphonatobenzyl)ammonium, sodium salt at 20 °C ± 0.01 °C (compared to water at 4 °C) is D420 = 1.30.

Description of key information

The relative density of Special Brilliant Blue FFR was determined according to OECD 109, which is in all relevant parts similar to EC A.3. The solid sample was accurately weighed into a 20 mL pycnometer and by displacement of the solvent (n-heptane), its equivalent volume was determined. Finally, the density of the sample was calculated (Opala 2017).

The relative density of Special Brilliant Blue FFR at 20 °C was determined as 1.273.

According to OECD Test Guidelines 109 (2012), Regulation EC No. 440/2008 Method A.3. and EPA OCSPP Test Guideline 830.7300 (2002), the relative density D420 of the test item Hydrogen [4-[[4-diethylamino)phenyl][4-[ethyl[(3-sulphonatobenzyl)amino]-o-tolyl]methylene]-3-methylcylohexa-2,5-dien-1-ylidene](ethyl)3-sulphonatobenzyl)ammonium, sodium salt at 20 °C ± 0.01 °C (compared to water at 4 °C) is D420 = 1.30 (Kremer 2018).

Key value for chemical safety assessment

Relative density at 20C:

1.3