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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge nitrification inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to guideline
Guideline:
ISO 9509 (Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms)
Version / remarks:
1988
Deviations:
not specified
GLP compliance:
no
Vehicle:
not specified
Details on test solutions:
- due to foam development of the substance a defoaming agent was added to the preparations
Test organisms (species):
activated sludge
Details on inoculum:
- Name and location of sewage treatment plant where inoculum was collected: OECD sludge, laboratory scale sawage treatment unit (10 L)
- Initial biomass concentration: 3 g/L dry weight
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
4 h
Hardness:
no data
Test temperature:
ca 25 °C
Nominal and measured concentrations:
50, 100, 500, 1000 mg/L nominal
Details on test conditions:
TEST SYSTEM
- Control: inoculum without Test substance
- Control : inoculum with reference inhibitor ATH (100 % inhibition)
- Sludge concentration (weight of dry solids per volume): 3 g/L dry weight

Reference substance (positive control):
no
Duration:
4 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of nitrification rate
Details on results:
- Nitrification rate: 2.6 mg/h*g oxidated N
Validity criteria fulfilled:
not specified
Conclusions:
Special Brilliant Blue FFR showed an EC 50 > 1000 mg/L after 4 hours (nitrification inhibition).
Executive summary:

A study was conducted according to ISO 9509 (Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms) to assess the toxicity of Special Brilliant Blue FFR towards microorganisms. The degree of inhibition on the nitrification performance of activated sludge was measured with several concentrations (50, 100, 500, 1000 mg/L nominal). A control with inoculum without test substance and a control with inoculum with reference inhibitor ATH (100 % inhibition) were run parallel. The temperature was ca. 25 °C. The nitrification rate was: 2.6 mg/h*g oxidated N.

Special Brilliant Blue FFR showed an EC 50 > 1000 mg/L after 4 hours (nitrification inhibition).

Description of key information

A study was conducted according to ISO 9509 (Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms) to assess the toxicity of Special Brilliant Blue FFR towards microorganisms. The degree of inhibition on the nitrification performance of activated sludge was measured with several concentrations (50, 100, 500, 1000 mg/L nominal). A control with inoculum without test substance and a control with inoculum with reference inhibitor ATH (100 % inhibition) were run parallel. The temperature was ca. 25 °C. The nitrification rate was: 2.6 mg/h*g oxidated N.

Special Brilliant Blue FFR showed an EC 50 > 1000 mg/L after 4 hours (nitrification inhibition).

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

Should read EC50 > 1000 mg/L