1,2,3-trichlorobenzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Version / remarks:

1981

Deviations:

yes

Remarks:

Analytical purity not reported. No GLP. Observation time too short

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,2,3-trichlorobenzene

EC Number:

201-757-1

EC Name:

1,2,3-trichlorobenzene

Cas Number:

87-61-6

Molecular formula:

C6H3Cl3

IUPAC Name:

1,2,3-trichlorobenzene

Test material form:

solid

Details on test material:

- Name of test material (as cited in study report): 1,2,3- trichlorobenzene (solidified melted)
- Physical state: solid
-Analytical purity: no data

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not reported
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storage: not reported
- Stability in the medium, i.e. sensitivity of the test material to hydrolysis and/or photolysis: not reported
- Solubility and stability of the test material in the solvent/vehicle and the exposure medium: not reported
- Reactivity of the test material with the incubation material used (e.g. plastic ware): not reported

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing (e.g. warming, grinding): prepared as a paste with water
- Final concentration of a dissolved solid, stock liquid or gel: not reported

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Hacking & Churchill ltd., Huntingdon, UK
- Weight at study initiation: 3 kg
- Diet (e.g. ad libitum): Futter "Ssniff K4" (Ssniff Versuchstierdiaten GmbH, Soest/Westf., Germany) ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-21°C Celsius
- Humidity (%): 40-50%
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs light (7 a.m.-7 p.m.)

IN-LIFE DATES: The test was conducted during April 1982

Test system

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

water

Remarks:

test subtance was prepared as a paste with water

Controls:

other: the untreated site served as control

Amount / concentration applied:

Concentration: 500 mg

Duration of treatment / exposure:

4 hour(s)

Observation period:

1, 24, 48 and 72 hours, 7 days

Number of animals:

3

Details on study design:

TEST SITE
- Area of exposure: The flank skin of the three animals was shaved on both sides with electric cutter on an area of about 6x6 cm (24 h before testing the intact skin)
- % coverage: not specified
- Type of wrap if used: gauze patch (2.5x2.5 cm).

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: 4 hours

OBSERVATION TIME POINTS
(indicate if minutes, hours or days): 24, 48, 72 hrs

SCORING SYSTEM:
- Method of calculation: according to Draize

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 2

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 3

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible within: 72 hrs

Results 4

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 5

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 6

Irritation parameter:

edema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0

Max. score:

4

Irritant / corrosive response data:

Additional symptoms were not observed

Any other information on results incl. tables

Evaluation results of skin reactions after 1,2,3-trichlorobenzene administration to rabbits

Readings	Animal	Erythema	Edema
24 h	68	0	0
24 h	69	0	0
24 h	70	1	0
48 h	68	0	0
48 h	69	0	0
48 h	70	1	0
72 h	68	0	0
72 h	69	0	0
72h	70	0	0
Mean24 -72h	68	0.0	0
Mean24 -72h	69	0.0	0
Mean24 -72h	70	0.3	0
Total mean		0.1	0

 

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Erythema score (mean 24/48/72 hrs per animal) = 0.0 / 0.0 / 0.3.
Oedema score (mean 24/48/72 hrs per animal) = 0.0 / 0.0 / 0.0.

Executive summary:

Suberg H (1982)

 

An acute dermal irritation/corrosion study was conducted according OECD Guideline 404 with deviations (Analytical purity not reported. No GLP. Observation time too short).

3 New Zealand White rabbits were exposed to 0.5 g 1,2,3 -trichlorobenzene for 24 hours. Observation time points were 1h, 24h, 48, 72h and 7 days after removal of the patch.

The mean erythema score at 24-72h after exposure was 0.3 for 1/3 of the animals tested, 0 for the other ones. The oedema score between was found to have a value of 0 at 24 -72h after exposure.

On the basis of the data obtained 1,2,3-trichlorobenzene can not be classified as irritant.