1,2,3-trichlorobenzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From: 12.1.1982 To: 2.2.1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

No analytical purity reported. No GLP. Only 2 doses tested.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,2,3-trichlorobenzene

EC Number:

201-757-1

EC Name:

1,2,3-trichlorobenzene

Cas Number:

87-61-6

Molecular formula:

C6H3Cl3

IUPAC Name:

1,2,3-trichlorobenzene

Test material form:

solid

Details on test material:

- Name of test material (as cited in study report): 1,2,3-trichlorobenzene solidified melted
- Physical state: solid
- Analytical purity: not reported

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not reported
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storage: not reported
- Stability in the medium, i.e. sensitivity of the test material to hydrolysis and/or photolysis: not reported
- Solubility and stability of the test material in the solvent/vehicle and the exposure medium: not reported
- Reactivity of the test material with the incubation material used (e.g. plastic ware): not reported

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing (e.g. warming, grinding): formulated in Lutrol
- Final concentration of a dissolved solid, stock liquid or gel: not reported

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Sparte, OC, Germany
- Age at study initiation: 9-14 weeks old
- Weight at study initiation: 182 (males), 177 (females)
- Fasting period before study: from 16 hours before the application to 4 hours after the application
- Housing: Makrolon cages type III
- Diet (e.g. ad libitum): ad libitum (except during the fasting period)
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1.5 ° C
- Humidity (%): 60 ± 5%
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs light (7 a.m-7 p.m)


IN-LIFE DATES: From: 12.1.1982 To: 2.2.1982

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Lutrol

Details on oral exposure:

VEHICLE
- Concentration in vehicle: not reported
- Amount of vehicle (if gavage): not reported
- Justification for choice of vehicle: not reported
- Lot/batch no. (if required): not reported
- Purity: not reported

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg bw

Doses:

3100 and 5000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: At the day of the treatment the animals were controlled several times. During the 14-day observation period they were monitored twice per day ( once per day over weekends and holidays). The body weights were recorded at the day of the treatment, after 1 week and the end of the observation period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, gross pathology

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality was observed in the male and female group dosed with 3100  mg/kg bw. 2/5 males and 2/5 females dosed with 5000 mg/kg bw died within the first  week post application.

Clinical signs:

bodyweight loss

Body weight:

other body weight observations

Gross pathology:

Gross autopsy of some of the surviving rats showed no pathological  findings.

Any other information on results incl. tables

Table 1.Effect of acute oral toxicity test

 

Doses	Toxicological results			Time of death	Start of the symptoms
mL/kg	Dead animals	Animals with symptoms	Total of animal employed
Males
3.1	0	3	5		7 d
5.0	2	5	5	2-3d	24 h
Females
3.1	0	4	5
5.0	2	5	5	2-6 d	24 h

 

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Combined LD50 > 5000 mg/kg bw/day.

Executive summary:

Löser E (1982)

 

In order to test the acute oral toxicity of 1,2,3 -trichlorobenzene, 5 male and 5 female tested were dosed by gavage (3100 and 5000 mg/kg bw) with a method similar to OECD guideline 401 with deviations (No analytical purity reported. No GLP. Only 2 doses tested)

 

The following toxicological effects were observed:

1) Mortality: no mortality was observed in the male and female group dosed with 3100  mg/kg bw. 2/5 males and 2/5 females dosed with 5000 mg/kg bw died within the first  week post application.

 

2) Signs of intoxication
Rats of both sex and in both dose groups suffered from weight loss, rough  fur, bloody snout and reduced general condition.

 

3) Gross autopsy 
Gross autopsy of some of the surviving rats showed no pathological  findings.