Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
cytotoxicity
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficient for assessment

Data source

Reference
Title:
No information
Author:
Fratello G. et al. (1997). Cytotoxicity of halogenated benzenes and its relationship with logP. Toxicology in Vitro 11, 673-677

Materials and methods

Principles of method if other than guideline:
The in vitro toxicity of a series of environmentally relevant halobenzenes was tested using a Chinese hamster lung fibroblast cell line and its relationship with the logarithm of octanol/water partition coefficient (logP) was investigated. Colony ability forming (CFA) was performed and the DNA demage was analyses by cytofluorimetric assay.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2,3-trichlorobenzene
EC Number:
201-757-1
EC Name:
1,2,3-trichlorobenzene
Cas Number:
87-61-6
Molecular formula:
C6H3Cl3
IUPAC Name:
1,2,3-trichlorobenzene

Results and discussion

Any other information on results incl. tables

There was a dose dependent increase in inhibition of NRU uptake from 0 % inhibition at 0.0001 mg/ml up to 92 % at 1 mg/ml. Colony-forming ability (CFA) test  was performed with V79 Chinese hamster lung cells that were treated with  0.075, 0.05, 0.025, 0.01 mg/ml for 24 hours (incubation time) and the  concentration that inhibited the CFA by 50 % was derived:  IC50 = 0.24 mM


 

Applicant's summary and conclusion