Registration Dossier
Registration Dossier
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EC number: 202-327-6 | CAS number: 94-36-0
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The 96h-LC50 value for the test substance is 0.0602 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.06 mg/L
Additional information
Three experimental results were available in order to assess the toxicity of Dibenzoyl peroxide to fish. However, these results were considered as unreliable due to the insufficient documentation or unsuitable test systems. As a consequence, a new test was performed according to EEC method C.1 (1984) and OECD Test Guideline 203 (1984) and GLP criteria.
These four studies are summarised below:
- K3 Supporting Study (significant methodological deficiencies: no analytical monitoring of the tested substance concentration Significant methodological deficiencies: no analytical monitoring of the tested substance concentration): dibenzoyl peroxide was tested in an acute toxicity test withPoecilia reticulate(guppy) under semi-static conditions in accordance with EEC method C.1 (1984) and OECD Test Guideline 203 (1984). Due to the insolubility of the test substance in water the test concentrations were prepared using acetone as an organic solvent. Each test concentration contained 100 µg/l of acetone, as did one control. The results of the test are expressed as pure active ingredient. The LC50-96 h a.s. calculated was 2.0 mg/1 with confidence limits of 1.7 mg/1 and 2.4 mg/1.
- K4 Supporting Study (insufficient documentation for assessment) : this experiment was performed following OECD Guidelines for Testing of Chemicals (1996), “Fish, Acute Toxicity Test (No. 203, adopted : July 17, 1992)” to identify the degree of acute toxicity of benzoyl peroxide on small fry using continuous flow-through system. The toxicology test was performed at the concentration of 0.23, 0.47, 0.69, 1.54 and 2.17 mg/L (mean measured concentration) using 10 small fry for each concentration. The number of the dead fish and sub-lethal effect was observed at 24, 48, 72 and 96 hours after exposure. LC50-96h was estimated at 0.24 mg/l.
- K4 study (documentation insufficient for assessment): the LC50 of dibenzoyl peroxide was estimated at 3.9 mg/l (MOE, 1996).
- K1 Key Study: results are summarised in table below:
Acute toxicity to fish
|
Initial mean measured Dibenzoyl peroxide (mg/L) |
EC50(96 h) |
0.0602 [0.0477, 0.0856] |
No observed effect concentration (NOEC) |
0.0316 |
[95% confidence limits]
Therefore, Dibenzoyl peroxide can be considered as very toxic to fish.
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