Registration Dossier
Registration Dossier
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EC number: 222-023-7 | CAS number: 3320-83-0
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Unsuitable test system
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
Test material
- Reference substance name:
- 2-chlorophenyl isocyanate
- EC Number:
- 222-023-7
- EC Name:
- 2-chlorophenyl isocyanate
- Cas Number:
- 3320-83-0
- Molecular formula:
- C7H4ClNO
- IUPAC Name:
- 1-chloro-2-isocyanatobenzene
- Details on test material:
- purity not specified
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - direct weight
- pH-value: 6.8
- concentration: 400.0 mg/L
Test organisms
- Test organisms (species):
- activated sludge, domestic
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 +/- 2 °C
- pH:
- - test substance: pH-value: 8.2 - 8.4
- reference substance: pH-value: 8.2 - 8.4 - Dissolved oxygen:
- Test substance:
- at 100 mg/L: 5.7 mg O2/L - 2.5 mg O2/L
- at 1000 mg/L: 6.0 mg O2/L - 2.7 mg O2/L
- at 10000 mg/L: 5.9 mg O2/L - 2.6 mg O2/L
reference substance:
- at 5 mg/L: 5.1 mg O2/L - 2.6 mg O2/L
- at 10 mg/L: 6.8 mg O2/L - 4.7 mg O2/L
- at 20 mg/L: 7.7 mg O2/L - 6.8 mg O2/L - Nominal and measured concentrations:
- - nominal concentrations: 100, 1000, 10000 mg/L
- Details on test conditions:
- The test substance concentrations were diluted with 130 mL dest. water and stirred over night.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- EC50 = 14 mg/L (Probit-Analysis)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
