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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
activated sludge nitrification inhibition testing
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
publication
Title:
A database of chemical toxicity to environmental bacteria and ist use in interspecies comparisons and correlations
Author:
Blum DJW and RE Speece
Year:
1991
Bibliographic source:
Research Journal WPCF 63(3): 198-207

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
ISO 9509 (Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2,3-trichlorobenzene
EC Number:
201-757-1
EC Name:
1,2,3-trichlorobenzene
Cas Number:
87-61-6
Molecular formula:
C6H3Cl3
IUPAC Name:
1,2,3-trichlorobenzene

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Nitrosomonas sp.

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
25°C
pH:
6.5-8.0
Details on test conditions:
- Test vessel: 125 mL serum bottle
- Liquid Volume: 50 mL
- Shaking: yes

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
IC50
Effect conc.:
96 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
other: Ammonia use

Applicant's summary and conclusion