Diisopropyl adipate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

the test solution was prepared by dissolving 90 µl, 98 µl, 110µl, 125 µl, 175 µl, 225 µl, 250 µl, 500 µl & 1 ml of the test substance in 10 liters of deionized water for achieving test concentrations of 9 mg/L, 9.8 mg/L, 11 mg/L, 12.5 mg/L, 17.5 mg/L, 22.5 mg/L, 25 mg/L, 50 mg/L& 100mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 2-2.5 cm
- Weight at study initiation (mean and range, SD): average 0.250 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12-hour darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

29°C

pH:

7.2

Dissolved oxygen:

7.4 mg/L

Nominal and measured concentrations:

Nominal Conentration: 9 mg/L, 9.8 mg/L, 11 mg/L, 12.5 mg/L, 17.5 mg/L, 22.5 mg/L, 25 mg/L, 50 mg/L & 100mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: of 12 hours light and 12-hour darkness

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

< 11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Other details not known

Sublethal observations / clinical signs:

Mortality and symptoms

Nominal conc. (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	0	0	0	0
9.0	8	0	0	0	0
9.8	8	0	0	0	0
11	8	8	-	-	-
12.5	8	8	-	-	-
17.5	8	8	-	-	-
22.5	8	8	-	-	-
25	8	8	-	-	-
50	8	8	-	-	-
100	8	8	-	-	-

 

 

 

 

Nominal conc. (mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
9.0	8	NS8	NS8	NS8	NS8
9.8	8	NS8	NS8	NS8	NS8
11	8	-	-	-	-
12.5	8	-	-	-	-
17.5	8	-	-	-	-
22.5	8	-	-	-	-
25	8	-	-	-	-
50	8	-	-	-	-
100	8	-	-	-	-

 

 

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. on Zebra Fish Danio rerio was determine to be <11 mg/L

Executive summary:

Study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test concentrations selected for the test was 9 mg/L, 9.8 mg/L, 11 mg/L, 12.5 mg/L, 17.5 mg/L, 22.5 mg/L, 25 mg/L, 50 mg/L & 100mg/L. Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be <11 mg/L. As the test chemical is readily biodegradable it cannot be classified as per CLP criteria.

Description of key information

Study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test concentrations selected for the test was9 mg/L, 9.8 mg/L, 11 mg/L, 12.5 mg/L, 17.5 mg/L, 22.5 mg/L, 25 mg/L, 50 mg/L & 100mg/L.Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be <11 mg/L. As the test chemical is readily biodegradable it cannot be classified as per CLP criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

11 mg/L

Additional information

Study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test concentrations selected for the test was9 mg/L, 9.8 mg/L, 11 mg/L, 12.5 mg/L, 17.5 mg/L, 22.5 mg/L, 25 mg/L, 50 mg/L & 100mg/L.Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be <11 mg/L. As the test chemical is readily biodegradable it cannot be classified as per CLP criteria.