2-ethoxyethylamine

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study in compliance with GLP-criteria.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-ethoxyethylamine

EC Number:

203-801-5

EC Name:

2-ethoxyethylamine

Cas Number:

110-76-9

Molecular formula:

C4H11NO

IUPAC Name:

2-ethoxyethylamine

Details on test material:

- Name of test material (as cited in study report): 2-Ethoxyethylamin
- Physical state: liquid
- Stability under test conditions: stable

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
A stock solution of 12500 mg/L was prepared by stirring the test substance in deionised water for 5 minutes. The test concentrations were made by diluting the stock solution with deionized water.

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: The used test strain of Pseudomonas putida DSM 50026 is purchased on regular times from DSM (Deutsche Sammlung von Mikroorganismen in Braunschweig / German collection of microorganisms in Braunschweig) and cultured further on agar slants in the Laboratory of Ecotoxicology of BASF AG in Ludwigshafen, Germany.
- Method of cultivation: The stock cultures are kept in slant agar culturing tubes of 50 mL. The inoculation density is one smear from an inoculating loop. The culture is transferred weekly.
- Pretreatment: The pre-cultures are kept in Erlenmeyer flasks with a nominal volume of 250 mL. The vessels are filled with 100 mL pre-culture medium and inoculated with a smear from an inoculation loop with 10 TE/F bacteria suspension at 21 ± 1 °C and incubated for 7 h while shaking.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test conditions

Test temperature:

21 ±1 °C

pH:

7.0 -10.8

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 19.53, 39.06, 78.13, 625, 625 (pH neutralised), 1250, 2500, 2500 (pH neutralised), 5000, 10000 and 10000 (pH neutralised) mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: closed tubes of glass with flat bottom (nominal volume 50 mL, fill volume: 10 mL) plugged with gas unpermeable teflone caps
- No. of organisms per vessel (inoculum density): 5 FNU
- No. of vessels per concentration (replicates): 4 inoculated replicates and 1 unicolated replicate
- No. of vessels per control (replicates): 4 inoculated replicates and 1 unicolated replicate

TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: yes
- Test medium: AK-Medium according to DIN 38412, Part 8

EFFECT PARAMETERS MEASURED
Optical density at a wavelength of 436 nm. Measurements are made at t=16h in all inoculated replicates, whereby the uninoculated replicate of each concentration is used as background measurement.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

82.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks:

(inhibition of cell multiplication)

Remarks on result:

other: non-neutralised test solutions

Effect concentrations 2

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

145 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks:

(inhibition of cell multiplication)

Remarks on result:

other: non-neutralised test solutions

Effect concentrations 3

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

750 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks:

(inhibition of cell multiplication)

Remarks on result:

other: neutralised test solutions

Details on results:

The inhibition of bacterial cell multiplication is most likely due to pH effects. The corresponding neutralised samples showed a significantly lower toxicity compared to the non-neutralised samples.

Any other information on results incl. tables

Optical densities of the bacterial cultures at t = 16 h and pH values of inoculated and uninoculated replicates

Concentration  (mg/L)	Optical density (mean, corrected)	Growth (%control)	Inhibition (% control)	pH
				uninoculated		inoculated
				0 h	16 h	(min - max)
Control	0.303	100.0	0.0	7.1	7.2	6.2 - 6.3
19.53	0.302	99.6	0.4	7.4	7.4	6.4 - 6.5
39.06	0.343	113.1	-13.1	7.6	7.7	6.4 - 7.0
78.13	0.284	93.8	6.2	8.8	8.6	6.3 - 6.4
156.25	0.136	44.8	55.2	9.3	9.2	6.2 - 6.4
312.5	0.007	2.1	97.9	9.7	9.5	9.4 - 9.5
625	0.007	2.0	98.0	9.9	9.7	9.8 - 9.8
1250	0.007	2.0	98.0	10.1	10.0	10.0 - 10.1
2500	0.007	1.7	98.3	10.3	10.2	10.2 - 10.3
5000	0.009	2.2	97.8	10.5	10.4	10.5 - 10.5
10000	0.017	2.2	97.8	10.8	10.7	10.7 - 10.8
625*	0.161	52.8	47.2	7.2	7.2	4.3 - 5.0
2500*	0.096	31.4	68.6	7.1	7.1	4.2 - 4.3
10000*	0.072	23.3	76.7	7.0	7.1	4.7 - 4.8