2-ethoxyethylamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DS/EN ISO 14669

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethoxyethylamine

EC Number:

203-801-5

EC Name:

2-ethoxyethylamine

Cas Number:

110-76-9

Molecular formula:

C4H11NO

IUPAC Name:

2-ethoxyethylamine

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:(nominial) 0, 20, 50, 100, 200. 500 and 1000 mg/l
- Sampling method: all testkoncentrations
- Sample storage conditions before analysis: Frozen at - 20 C right after sampling

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other aquatic crustacea: Acartia tonsa

Details on test organisms:

TEST ORGANISM
- Common name: Calanoid copepod (marine)
- Strain: Kept in culture at DHI since 1987, originally collected in the North Sea by Danmarks Fiskeriundersøgelser

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.3 ± 0.1 ºC

pH:

8.0 ± 0.2

Dissolved oxygen:

≥ 4 mg/l at test end

Salinity:

31.8 ‰

Nominal and measured concentrations:

Nominal concentrations: 0, 20, 50, 100, 200. 500 and 1000 mg/l
Analytical control over time of nominal concentrations
Time(hours) Nominal concentration (mg/l) Actual measured koncentration (mg/l)
0 1000 800
0 500 550
0 200 170
0 100 86
0(Control) 0 <0.1
48 1000 830
48 500 580
48 200 180
48 100 84
48(Control) 0 <0.1

Details on test conditions:

TEST SYSTEM
- Test vessels: Glass beakers with approx. 25 ml of test soulution

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
-

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (light:dark)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 211 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC10

Effect conc.:

ca. 112 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: yes
- LC50: 0.58 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50(48 h) of 2-ethoxyethylamine, CAS No.110-76-9, is above 211 mg/L for Acatia tonsa