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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: OSPAR Commission 2006: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry (Part B was amended in 2006 (OSPAR summary record 06/23/1, §9.7))
Deviations:
yes
Remarks:
Slightly smaller fish was used(3.55 ± 0.26 cm) than recomended in guideline (4-6 cm). This is considered to be of minor importance.
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
Modified according to OSPAR Commission 2006: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-ethoxyethylamine
EC Number:
203-801-5
EC Name:
2-ethoxyethylamine
Cas Number:
110-76-9
Molecular formula:
C4H11NO
IUPAC Name:
2-ethoxyethylamine
Test material form:
other: liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations:(nominial) 0, 50, 100, 200. 500 and 1000 mg/l
- Sampling method: 40 ml taken out in 50 ml bottles of all testkoncentrations
- Sample storage conditions before analysis: Frozen at - 20 C right after sampling

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Scophtalmus maximus
Details on test organisms:
TEST ORGANISM
- Common name: Turbot
- Source: Maximus in Thisted, Denmark
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): 3.56 ± 0.29 cm
- Weight at study initiation (mean and range, SD): 1.07 ± 0.2 g

Study design

Test type:
static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Test temperature:
15.4 ± 0.2 ºC
pH:
7.9 - 0.1
Dissolved oxygen:
>60 %
Salinity:
31.6 - 33.9 ‰
Nominal and measured concentrations:
Nominal concentrations: 0(control), 50, 100, 200, 500 and 1000 mg/l.
Analytical control over time of selected nominal concentrations:
Time(hours) Nominal concentration (mg/l) Actual measured koncentration (mg/l)
0 (Control) 0 <0.1
24 (Control) 0 <0.1
48 (Control, old) 0 <0.1
48 (Control, new) 0 <0.1
72 (Control) 0 <0.1
96 (Control) 0 <0.1
0 1000 870
24 1000 860
48 (old) 1000 900
48 (new) 1000 860
72 1000 860
96 1000 880
Details on test conditions:
- Test vessels: 20 l aquariums

- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: approx 1 g per l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
-

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (light:dark)

Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Duration:
96 h
Dose descriptor:
LC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Mortality: yes
- LC50: 0.6 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50(96 h) of 2-ethoxyethylamine, CAS No.110-76-9, is above 1000 mg/L for Scophtalmus maximus