Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways


Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

DT50 pH 4 (25 °C) = 26 hours
DT50 pH 7 (25 °C) = 219 hours
DT50 (25 °C) = 1147 hours

Key value for chemical safety assessment

Additional information

The abiotic degradation of ethyl 3,5 -dichloro-4 -hexadecyloxybenzoate (AF-366) via hydrolysis was investigated by Rhemrev-Boom (1989) and Rhemrev-Boom and Houtzager (1992). The first study was a preliminary test conducted according to OECD 111 under GLP conditions. The test substance was incubated for 5 days at 50 °C in three different buffered solutions, at pH 4, pH 7 and pH 9. The results of the test showed that > 10% hydrolysis took place in all three solutions (30% at pH 4, 39% at pH 7 and 49% at pH 9) and therefore, further testing was needed in order to determine the precise abiotic degradation of the substance.


The second (main) test was performed according to the Council Directive 67/548/EEC method “Hydrolysis as a function of pH”. A preliminary study was carried out for 44 hours, after which it was deemed necessary to continue with the full test. The test substance was incubated at pH 4, 7 and 9 at two different temperatures (25 °C and 50 °C). The entire test was conducted under sterile conditions and the temperature controlled within 0.5 °C of the nominal temperatures. Under environmentally relevant conditions (25 °C) the half-lives of the substance were 26 hours, 219 hours and 1147 hours at pH 4, pH 7 and pH 9, respectively.


These values indicate that ethyl 3,5 -dichloro-4 -hexadecyloxycarbonyloxybenzoate (AF-366)

can be expected to be rapidly degraded abiotically in water at pH 4 and pH 7, but is considered to be hydrolytically stable at pH 9.