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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Description of key information

72h-EC50 > 100 mg/L (nominal)
72h-EC50 > 0.078 mg/L (limit water solubility)

Key value for chemical safety assessment

Additional information

A study investigating the toxicity of ethyl 3,5 -dichloro-4 -hexadecyloxycarbonyloxybenzoate (AF-366) in algae was conducted by Bruns (2003). This test was performed according to EU Method C.3, method equivalent to OECD Guideline 201, under GLP conditions.Desmodesmus subspicatus was exposed to a single concentration of 100 mg/L (nominal, filtered solution (7-12 µm)) for 72 hours (3 replicates). Additionally, one replicate was included in the experimental set-up that contained test substance, but no algae. Analytical measurements of actual test concentrations were not performed.


No adverse effects on algal biomass or growth rate were reported after the 72-hour exposure period. However, a slight hormetic effect could be observed when comparing mean cell growth and cell growth rates with those in the controls, with an increase of 17.3% and 2.9% respectively, which however were not significant. Therefore, the 72h-EC50 was > 100 mg/L (nominal).


In conclusion, AF-366 is not toxic to algae within the range of its water solubility (≤ 0.078 mg/L).