D-Glucopyranose, oligomeric, C10-16-alkyl glycosides, 2-hydroxy-3-sulfopropyl ethers, sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

48 hours

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Chinese GLP equivalent method

Deviations:

not applicable

Principles of method if other than guideline:

The objective of the study was to assess acute toxicity of the test item towards Daphnia magna (Daphnia magna Straus) under semi-static exposure procedure. The nominal concentrations of test item was 4.0,6.0,8.9. 13.4 and 20.0 mg/L, and blank control was set in parallel" Four replicates was set in each group and five daphnids were used in each replicate. The test duration lasted for 48 hours. lmmobilization was observed at 24 h and 48 h of exposure. The EC5s and its 95% confidence limits was calculated.
The test item is a UVCB substance, the change of the test item was monitored by total organic carbon (TOC) analysis during the test. The samples of the test item treatment groups and blank control group were taken to determine the TOC concentration at the beginning of exposure (0h), at the end of exposure (48h) and 24 hours before and after test solution renewal.

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

yes

Remarks:

water

Details on test solutions:

Based on the result of the preliminary test, the nominal concentrations of the test item were 4.0, 6.0, 8.9, 13.4 and 20.0 mgil in the definitive test.

Test organisms (species):

Daphnia magna

Details on test organisms:

Test system: Daphnia magna Straus
Source: The species Daphnia magna was originally supplied by Shenyang Research Institute of Chemical Industry. The clone has been breeding in CTI-SET.
Result of sensitivity lest: The result of the latest sensitivity test (Study No.:17ST003) conducted from 03 March 2017 to 05 March 2017 indicated that the 24h-EC5s of potassium bichromate (CAS No.: 7778-5A-q b Daphnia magna Straus was 1.0 mg/L and its 95%
confidential limit was 0.9-1.1 mg/L, which satisfied the requirement recommended by ISO 6341-2412 ffhe 24h-E0so of potassium bichromate ta Daphnia magna Straus was in the range of 0.6-2.1 rng/L).
Acclimation: the tap water dechlorinated by aeration was used to maintain the stock anirnals. During the acclimation, the temperature was controlled between 18-22oC, the pH values were 6-9, the oxygen concentration was above 3 mg/L. The test water were
inconsistent with that used in the culture, therefore the brood daphnids were maintained in test water under the test condition for 48 hours prior to the test. The test organisms were less than 24 hours old and the third brood progeny. They were derived from a healthy stock {the internal number of parent daphnids was Daphnia magna Q2017A6AT).

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

The hardness of test waterwas 240 mg /L (Calculate as CaCOa)

Test temperature:

The temperature of the test water was maintained at 20.67-20.91"C.

pH:

the pH value of test sofution was 7.71-8.04.

Dissolved oxygen:

During the period of the exposure, the dissolved oxygen was in the range of 8.53 - 8.80 mg/L

Nominal and measured concentrations:

The change of the test ilem was monitored by TOC analysis during the test. The TOC concentrations of test solution test item treatment groups and control group were determined at the beginning of exposure (0h), at the end of exposure (4Sh) and 24 hours before and after test solution renewal.
During the test, except the group of 4.0 mgll, the measured TOC concentration of test item in the solution was maintained in the range of 88%-104% of initial measured concentration, which was in the range of 80%-120%. The measured TOC concentration of test solution in the group of 4.0 mg/L was 62%-76% of initial measured concentration. lt was refened that the measured TOC concentration of 4.0 mg/L was low, which was in the range of 0.5 - 1.0 mg/L), and the TOC concentration of blank control solution was near the limit of detection (0.5 mg/L). Corrected by blank, the calculated TOC concentration of test item had a big fluctuation and the result did not indicate that the TOC concentration of test item could not be maintained during the test. Therefore, the effect concentration could be calculated based on the nominal concentration.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

12.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Based on the nominal concentrations, the 48h-EC50 of the test item to Daphnia magna was cafculated to be 12.9 mg/L and its 95% confidence limits was 11.4 - 14.7 mg/L.

Validity criteria fulfilled:

yes

Executive summary:

The objective of the study was to assess acute toxicity of the test item towards Daphnia magna (Daphnia magna Straus) under semi-static exposure procedure. The nominal concentrations of test item was 4.0,6.0,8.9. 13.4 and 20.0 mg/L, and blank control was set in parallel" Four replicates was set in each group and five daphnids were used in each replicate. The test duration lasted for 48 hours. lmmobilization was observed at 24 h

and 48 h of exposure. The EC50 and its 95% confidence limits was calculated.

Corrected by blank, the calculated TOC concentration of test item had a big fluctuation and the result did not indicate that the TOC concentration of test item could not be maintained during the test. Therefore, the efiect concentration could be calculated based on the nominal concentration.

Based on the nominal concentrations, the 48h-EC50 of the test item to Daphnia Magna was calculated to be 12.9 mg/L and its 95% confidence limits was 11.4-14.7 mg/L.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

other: ESA SOP 101 based on USEPA (2002)

Deviations:

no

GLP compliance:

yes

Test organisms (species):

Ceriodaphnia sp.

Test type:

static

Water media type:

not specified

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 16.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

0%

Conclusions:

EC50(48H) = 16.3 mg/l
NOEC= 10mg/l
LOEC=20mg/l

Executive summary:

48h Acute (survival) toxicity test using the freshwater cladoceran (Ceriodaphnia of dubia)

Method: ESA SOP 101 , based on USEPA (2002)

No deviation

Test temperature 25 +/- 1ªC

Test performed under GLP Principles by ECOTOX Services Australia

NOEC: 10 mg/l

Description of key information

Based on the nominal concentrations, the 48h-EC50 of the test item to Daphnia Magna was calculated to be 12.9 mg/L and its 95% confidence limits was 11.4-14.7 mg/l, based on immobility

In addition, 48h Acute (survival) toxicity test using the freshwater cladoceran (Ceriodaphnia of dubia) gave a value of 16.3 mg/l, based on mortality

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

12.9 mg/L