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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Version / remarks:
SOP E224 based upon OECD Guidelines for Testing of Chemicals Ready Biodegradability reference 301D
Principles of method if other than guideline:
The test vessels are 250ml BOD bottles filled to absolute volume with the test substance dissolved in the test medium, then sealed with a glass stopper. The bottles are incubated in the dark at 22±2°C and the degradation is followed by analysis of the dissolved oxygen over a 28 day period.
GLP compliance:
yes
Specific details on test material used for the study:
Identification:
Name as supplied: SugaNate 160NC D-Glucopyranose, oligomeric, C10-16-alkyl glycosides, 2-hydroxy-3-sulfopropyl ethers, sodium salts SugaNate 160NC
Batch number: 58474H17 (C of A)
Purity / Composition: Sodium Laurylglucosides Hydroxypropylsulfonate,~33% active, 39.8% solids (CofA), CAS No. 742087-49-6 (SDS)
Chemex reference: ECO 171011
Appearance (observed): Clear liquid, Clear yellow liquid
Solubility (specified): Soluble
The test material was stated to be soluble in water, therefore a stock solution of 0.2000g of SugaNate 160NC D-Glucopyranose, oligomeric, C10-16-alkyl glycosides, 2-hydroxy-3-sulfopropyl ethers, sodium salts. was made to 100ml with deionised water to produce a concentration of 2.0g/l.
Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic (adaptation not specified)
Details on inoculum:
The inoculum used for this test was the secondary effluent from a predominantly domestic sewage treatment works.
Inoculum source: Cambridge STW, Cowley Road, Cambridge
Date of collection: 15 June 2018
Pre-treatment: Filtered (Whatman No. 54)
Temperature at start of test: 20.8°C
Concentration of inoculum: 1ml/l
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation:
O2 consumption
Remarks:
BOD
Reference substance:
acetic acid, sodium salt
Parameter:
% degradation (O2 consumption)
Value:
84
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
97
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
94
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
85
Sampling time:
28 d
Details on results:
Values are for a test concentration of 11.00mg/l and a Chemical Oxygen Demand (COD) of 424mgO2/g.
The degradation is defined as the ratio of the biochemical oxygen demand (BOD) within 28 days to either the theoretical oxygen demand (ThOD) or the chemical oxygen demand (COD).

SugaNate 160NC D-Glucopyranose, oligomeric, C10-16-alkyl glycosides, 2-hydroxy-3-sulfopropyl ethers, sodium salts gave a positive result (>=60% degradation relative to the COD value) with 84% being reached by day 7 and a maximum of 97% achieved on day 14. It may therefore be concluded that there is a potential for biodegradation under environmental conditions.

The guideline requires that the reference material degrades by at least 60% of its ThOD, and that the blank dissolved oxygen loss does not exceed 1.5mg/l after 28 days. Both of these criteria were satisfied for this test; a maximum value of 105% degradation of the sodium acetate was

achieved on day 14, and the blank DO loss was 1.36mg/l recorded on day 28. The replicate difference of extremes is also required to be less than 20% at the end of the test, the variation recorded was only 8.9%.

As the empirical formula was unavailable for the calculation of the Theoretical Oxygen Demand (ThOD), the Chemical Oxygen Demand (COD) was determined experimentally. It should be noted that the use of COD, as a reference value for degradation, is less satisfactory than the

ThOD as some chemicals are not fully oxidised in the COD test. Incomplete oxidation values will artificially increase the biodegradation value recorded.

The final conclusion of this study is that SugaNate 160NC D-Glucopyranose, oligomeric, C10-16 -alkyl glycosides, 2-hydroxy-3-sulfopropyl ethers, sodium salts is readily biodegradable under the conditions of this test.

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The final conclusion of this study is that SugaNate 160NC D-Glucopyranose, oligomeric, C10-16 -alkyl glycosides, 2-hydroxy-3-sulfopropyl ethers, sodium salts is readily biodegradable under the conditions of this test.
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD 301 and OECD 301E
Qualifier:
equivalent or similar to guideline
Guideline:
ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))
Deviations:
not specified
GLP compliance:
not specified
Remarks:
Not detailed in the report. The work was carried out in Australia
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Duration of test (contact time):
28 d
Initial conc.:
40 mg/L
Based on:
DOC
Parameter:
% degradation (DOC removal)
Value:
> 80 - < 82
Sampling time:
28 d
Details on results:
PRODUCT READY BIODEGRADABLE
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The product exceed 70% biodegradability requierement for ready biodegradability of a single organic substnce or antural product when tested according AS4351 Part 2.

Description of key information

OECD "Ready biodegradability" test 301D - Ready Biodegradability (Closed Bottle test)

Substance is readily biodegradable showing 84% degradation after 7 days and 85% degradation after 28 days in marine water.

It is also shown to be readily biodegradable in fresh water by method OECD 301E

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
marine water

Additional information

The substance " SugaNate 160" was evaluated for its biodegradability in accordance with an standard : AS4351 Part 2 "Determination by analysis of dissolved organic carbon (DOC)" which is equivalent to ISO 7827 -1994 and it is based on OECD "Ready Biodegradability" test 31 and 301E.

The product achieved 80 -82% biodegradability and exceeded the 70% biodegradability requirement for ready biodegrability of a single substance or natural product when tested according to AS4351 Part 2.