Registration Dossier
Registration Dossier
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EC number: 223-228-4 | CAS number: 3775-90-4
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The EC50 of Daphnia magna exposed Methacrylic acid tert-butylaminoethyl ester was established to >64.8 with the method C2 of the European Directive 92/69/CEE under GLP condition (reliability 1).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 64.8 mg/L
Additional information
The acute toxicity (inhibition of mobility) of METHACRYLIC ACID tert-BUTYLAMINOETHYL ESTER (METHACRYLATE DE TERTIOBUTYL AMINOETHYLE) for Daphnia magna was assessed according to the method C2 of the European Directive 92/69/CEE. The study was carried out in compliance with the Principles of OECD Good Laboratory Practices.
Daphnia were exposed in a static test to a range of nominal concentration of 1.56 to 200 mg/L, forming a geometric progression with a factor of 2. The test was performed with 20 daphnids per concentration. For each exposure concentration, the percentage of immobilization at 24 hours and 48 hours was recorded. The test concentrations of METHACRYLIC ACID tert BUTYL AMINOETHYL ESTER were measured by gas chromatography.
At the highest test concentration of 64.8 mg/l (geometric mean of the concentrations measured at the beginning and the end of the test), no daphnia was immobilized after 24 hours and 45 % of daphnids were immobilized after 48 hours of exposure. The EC50 -24h and EC50 -48h are both higher than 64.8 mg/L.
The method was applied with respect to the following quality criteria: immobilization in the control did not exceed 10 % at the end of the test; concentration of dissolved oxygen in the test vessels remained above 2 mg/L at the end of the test.
Two quality criteria were not respected: the pH varied by more than 1 unit at the two highest concentrations and the concentrations of the test subsbtance were not maintained within 80 % of the initial concentration throughout the duration of the test. The test concentrations remaining after 48 hours were in the range of 12 to 28 % of the initial concentration.
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