Disodium 1-amino-4-[[4-[(2-bromo-1-oxoallyl)amino]-2-sulphonatophenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 June, 1993 to 24 June, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

1. Deionised water instead of distilled water for the stock solution, 2. Preparation of the inoculum : higher sludge weight after the washing procedure, 3. 4 instead of 5 test concentrations, 4. The temperature of the test was 22.2 °C instead of 20±2 °C.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Code number: FAT - 92349/A
- Source and batch No.of test material: 56
- Expiration date of the batch: November 1997

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature, protected from light.
- Solubility and stability of the test substance in water: 100 g/L

OTHER SPECIFICS:
- Purity: 68 %
- Appearance: solid

Analytical monitoring:

not specified

Vehicle:

yes

Test organisms (species):

activated sludge of a predominantly industrial sewage

Details on inoculum:

- Organism: Activated sludge of a communal sewage treatment plant (ARA-Birsig Therwil)
- Sludge pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

22.2 "C

pH:

7.2 before the test beginning

Dissolved oxygen:

0.1 - 0.2 L/min by pasteur-pipette

Nominal and measured concentrations:

320,100, 32 and 10 mg/L (nominal concentrations)

Details on test conditions:

Experimental conditions:
Sludge dry weight: 1.56 g/L,
Test volume: 100 mL,
Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode system: OXI DIGI 530 (SAV: 00 109),
Aeration: 0.1 - 0.2 L/min by pasteur-pipette,
pH of sludge: 7.2 before the test beginning.

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol (Merck 821768)

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

The IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 7 mg/L

Reported statistics and error estimates:

IC50: The respiration rate of each test concentration was calculated as a percentage of the mean of the two control respiration rates. The results were plotted on a logarithmic (concentration)/normal (% inhibition) graph, where the IC50 can be evaluated.

Validity criteria fulfilled:

yes

Conclusions:

The IC50 (3 h) of the test substance FAT 92349/A was determined to be >320 mg/L.

Executive summary:

The toxicity of FAT - 92349/A to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline 209 and EG Method Nr. L 133/118. The test substance concentrations tested were 320,100, 32 and 10 mg/L. The reference substance ( 3,5 Dichlorophenol) concentration was 32, 10 and 3.2 mg/L. Based on the findings of the study, the IC50 (3 h) of the test substance FAT 92349/A was determined to be >320 mg/L. The IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 7.0 mg/L.

Description of key information

The toxicity of FAT - 92'349/A to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline 209 and EG Method Nr. L 133/118. The test substance concentrations tested were 320,100, 32 and 10 mg/L. The reference substance ( 3,5 Dichlorophenol) concentration was 32, 10 and 3.2 mg/L. Based on the findings of the study, the IC50 (3 h) of the test substance FAT 92349/A was determined to be >320 mg/L. The IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 7.0 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

320 mg/L