Disodium 1-amino-4-[[4-[(2-bromo-1-oxoallyl)amino]-2-sulphonatophenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 August, 1993 to 08 December, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Code number: FAT 92349/A
- Source and batch No.of test material: 56
- Expiration date of the batch: November 1997

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature, protected from light.
- Solubility and stability of the test substance in water: 100 g/L

OTHER SPECIFICS:
- Purity: 68 %
- Appearance: Solid

Analytical monitoring:

yes

Details on sampling:

The concentration of the test substance at the beginning and at the end of the test was analysed by the Analytical Services K, Textile Dyes Division.
All test medium samples for the analytical determinations were taken after mixing of the test solutions from the approximate center of the test vessels and kept at -18 °C to -25 °C until analyses.

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- STOCK SOLUTION:
0.5 g Test substance were mixed with 200 mL test medium and fill up to 500 mL with test medium. The stock solution was homogenized for five minutes in the supersonics bath.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: Laboratories of the Textile Dyes Division, Product Ecology TF
- Age of animals: Animals with age of less than 24 hours were used

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

136 mg/L CaCO3

Test temperature:

21.5 °C (start) , 21.2 °C (end)

pH:

8.0 (start), 7.8 to 7.9 (end)

Dissolved oxygen:

saturated (start), 96 - 99 % (end)

Nominal and measured concentrations:

The nominal concentrations tested were 10, 18, 32, 58, 100 and 180 mg/L and a control without any additions.

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL Beakers
- Material, size, headspace, fill volume: 100 - 200 mL test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Bidistilled water

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark

EFFECT PARAMETERS MEASURED: visual assessment of mobility of the daphnids after 24 and 48 hours.

VEHICLE CONTROL PERFORMED: yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 194.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

During test duation the test article concentrations in the analysed test media were in the range of 101.2 -109.0 % of the nominal values. Therefore under the test conditions the test article was sufficiently stable.
In all test concentrations no alteration of the test substance after 48 hours was observed. In the control and in the test concentrations up to 107.3 mg/L no immobilization of the daphnids was observed after 48 hours. Therefore, EC50 value could not be calculated. The EC0 value is at least ≥ 107.3 mg/L. The EC50 and EC100 values are higher than 194.6 mg/L.

Results with reference substance (positive control):

Potassium dichromate (EC50: 0.96 mg/L)

Validity criteria fulfilled:

yes

Conclusions:

The EC50 values were reported to be higher than 194.6 mg/L.

Executive summary:

The acute toxicity of FAT 92349/A to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline 202 and EU Method C.2. The nominal concentrations tested were 0 (Control), 10, 18, 32, 58, 100 and 180 mg/L. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 101.2 - 109.0 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable. In all test concentrations no alteration of the test substance after 48 hours was observed. In the control and in the test concentrations up to 107.3 mg/L no immobilization of the daphnids was observed after 48 hours. Therefore, EC50 value could not be calculated. Hence, the EC0 value is at least 107.3 mg/L and the EC50 values were reported to be higher than 194.6 mg/L.

Description of key information

The acute toxicity of FAT 92349/A to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline 202 and EU Method C.2. The nominal concentrations tested were 0 (Control), 10, 18, 32, 58, 100 and 180 mg/L. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 101.2 - 109.0 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable. In all test concentrations no alteration of the test substance after 48 hours was observed. In the control and in the test concentrations up to 107.3 mg/L no immobilization of the daphnids was observed after 48 hours. Therefore, EC50 value could not be calculated. Hence, the EC0 value is at least 107.3 mg/L and the EC50 values were reported to be higher than 194.6 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

194.6 mg/L