2-Propenoic acid, 2-fluoro-, methyl ester

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29/4/15 to 19/5/15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Recently conducted study performed to OECD guideline and under GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 10, 18, 32, 56, 100 mg/L
- Sampling method: 0h from bulk preparation, 48h from pooled replicates
- Sample storage conditions before analysis: frozen

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: No data
- Source: No data
- Age at study initiation (mean and range, SD):<24h
- Feeding during test: not fed

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

No data

Test temperature:

19 - 21oC

pH:

7.3 - 7.8 after 48 hours

Dissolved oxygen:

7.8 - 8.9 mg/L = >60% after 48 hours

Salinity:

No data

Nominal and measured concentrations:

Nominal concentrations (mg/L): 0 (control), 10, 18, 32, 56 100; Analytical concentrations (mg/L): 0 (control), 6.4, 12, 21, 39, 76

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL conical flask
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass, no headspace, containing 100 mL test medium
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): None - static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- Biomass loading rate: No data

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (ISO medium)
- Total organic carbon: No data
- Particulate matter: No data
- Metals: No data
- Pesticides: No data
- Chlorine: No data
- Alkalinity: No data
- Ca/mg ratio: No data
- Conductivity: No data
- Culture medium different from test medium: Elendt M7
- Intervals of water quality measurement: No data

OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 24h Dark
- Light intensity: Not applicable

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC0 (mobility)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 0.10, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: Yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

<= 47 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

<= 21 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Immobilisation forDaphnia magna(initial population 20)
Nominal concn (mg/L)	Mean measured concn (mg/L)	Observation period
		24-hour		48-hour
		Absolute no. immobilised	Cumulative no. immobilised	Absolute no. immobilised	Cumulative no. immobilised
Control (0)	<LoQ	0	0	0	0
10	6.4	0	0	0	0
18	12	0	0	0	0
32	21	0	0	0	0
56	39	1	1	0	1
100	76	0	0	20	20

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 value of the test substance was determined to be 47 mg/L, based on geometric measured concentrations.

Executive summary:

The 48–hour-acute toxicity of 2-propenoic acid to Daphnia magna was studied under static conditions. The study was conducted in accordance with EU method C.2 and OECD guideline 202, in compliance with GLP. 

Daphnids were exposed to a control and nominal test concentrations of 10, 18, 32, 56 and 100 mg/L for 48 hours.  Analytical measurements at 0 and 48 hour confirmed that the test concentration was not maintained within ± 20% of nominal. Immobilization and sub-lethal effects were observed daily.  The test results are expressed in terms of geometric mean measured concentrations. The 48–hour EC₅₀value was 47 mg/L.  The 48–hour NOEC based on immobilization was 21 mg/L.  There were no sub-lethal effects observed.

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

 

Results Synopsis

Test Organism Age: 1^stinstar (<24h)

Test Type: Static

EC₅₀: 47mg/L (95% C.I. could not be calculated)

Description of key information

The Key Study reported a 48 hour EC50 value for the toxicity of 2 -propenoic acid, 2 -fluoro, methyl ester to the freshwater invertebrate Daphnia magna of 47 mg/L based on geometric measured concentrations (Sacker, 2015).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

47 mg/L

Additional information

The 24 and 48 hour median effective concentrations (EC50) of 2-propenoic acid, 2-fluoro, methyl ester to the freshwater crustaceanDaphnia magna(<24 hours old) were determined under static test conditions at a temperature of 20±1°C. The EC50 was defined as the concentration calculated from the data that resulted in immobilisation of 50% of theDaphniain the time period specified. Based on geometric mean measured test solution concentrations the 48-hour EC50 was 47 mg/L (no 95% CIs) and the 48-hour NOEC was 21 mg/L. No symptoms of toxicity (sub-lethal effects) were observed.