2-Propenoic acid, 2-fluoro-, methyl ester

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13/4/14 to 11/5/15

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Qualifier:

equivalent or similar to guideline

Guideline:

other: US EPA Fate, Transport and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (q))

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent of a domestic sewage treatment plant (Loughborough)
- Storage conditions: Aerated at 20 ± 1oC
- Pre-treatment: Separation of coarse particles by filtration
- Concentration of sludge: 1% v/v

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Incubation temperature: 23 ± 1oC

Preliminary study:

No data

Test performance:

No data

Parameter:

% degradation (CO2 evolution)

Value:

21

Sampling time:

7 d

Parameter:

% degradation (CO2 evolution)

Value:

29

Sampling time:

14 d

Parameter:

% degradation (CO2 evolution)

Value:

33

Sampling time:

21 d

Key result

Parameter:

% degradation (CO2 evolution)

Value:

35

Sampling time:

28 d

Results with reference substance:

% degradation of reference substance (aniline)
14% degradtion after 7-d
70% degradation after 14-d
89% degradation after 21-d
99% degradatino after 28-d

Day	% Degradation
	Test substance	Reference substance	Toxicity control
7	21	14	9
14	29	70	61
21	33	89	70
28	35	99	72

Interpretation of results:

other: Not readily biodegradable

Conclusions:

The substance was not readily biodegradable under the employed test conditions

Executive summary:

A study was performed to assess the ready biodegradability of test substance according to Directive 92/69/EEC, method C.4 -D (Determination of 'ready' biodegradability - Manometric respiration test) and the equivalent OECD guideline 301F, in compliance with GLP.

The test substance in a mineral medium was inoculated with secondary domestic effluent and incubated for 28 d under aerobic conditions. During this period the biodegradation was followed by percentage BOD (CO₂evolution) based on the initial nominal concentration present.

The test substance showed 21, 29, 33 and 35 % degradation after 7, 14, 21, 28 d. Therefore, the test substance is classified as not readily biodegradable. The reference substance showed 70 % degradation after 14 d. The toxicity control showed 61% degradation after 14 d.

Under the employed test conditions, the test substance is considered not to be readily biodegradable. In addition, the test substance was not considered to have an inhibitory effect on the sewage treatment process microorganisms at the test concentration of 100 mg/l.

Description of key information

A reliable study (Klimisch 1) assessed biodegradability using a standard method equivalent to OECD guideline 301F, in compliance with GLP.

After 28 days a maximum of 35% degradation was observed, therefore, the test substance should be considered as not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable, not fulfilling specific criteria

Additional information

The test substance in a mineral medium was inoculated with secondary domestic effluent and incubated for 28 d under aerobic conditions. Biodegradation was determined by percentage BOD (CO2 evolution) based on the initial nominal concentration present. The test substance showed 21, 29, 33 and 35 % degradation after 7, 14, 21, 28 days, respectively. Therefore, the test substance should be considered as not readily biodegradable.