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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

additional ecotoxicological information
Type of information:
experimental study
Adequacy of study:
other information

Data source

Reference Type:
Acute toxicological effects of dimethyl sulfoxyde on the rough-skinned newt (Taricha granulosa)
Lappenbush WL and Willis DL
Bibliographic source:
Toxicology and Applied Pharmacology, 18, 141-150

Materials and methods

Test material

Constituent 1
Chemical structure
Reference substance name:
Dimethyl sulfoxide
EC Number:
EC Name:
Dimethyl sulfoxide
Cas Number:
Molecular formula:
dimethyl sulfoxide

Results and discussion

Any other information on results incl. tables

Species: amphibians

News were collected during the summer and spring, acclimated for 10 days at 10°C, weighed, treated with DMSO and maintained in an unfed state which simulated winter conditions.

Intraperitoneal injections of 15 g DMSO/kg or immersion in 2% DMSO were determined to be lethal threshold doses for newts. Death was rapid.

In addition to a transient increase in the body weight of the DMSO-treated animals, other changes were observed:
- a reduction in respiration to 70% that of the controls
- an increase in the weights of spleen (by day 1, the average spleen weight was over twice that of the controls), liver (33% increase after 24 h), and abdominal fat (150% increase after 20 days).

Lethality was attributed primarily to irreversible changes in the permeability of the skin, causing a water imbalance, hyperhydratation and death. The reduced respiratory rate increased the mortality rate

Applicant's summary and conclusion