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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
A toxicologic study of dimethyl sulfoxide
Author:
Willson JE, Brown DE and Timmens EK
Year:
1965
Bibliographic source:
Toxicol. Appl. Pharmacol. 7: 104-112

Materials and methods

GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Dimethyl sulfoxide
EC Number:
200-664-3
EC Name:
Dimethyl sulfoxide
Cas Number:
67-68-5
Molecular formula:
C2H6OS
IUPAC Name:
dimethyl sulfoxide

Test animals

Species:
mouse
Strain:
other: albino
Sex:
male/female

Administration / exposure

Route of administration:
intravenous
Vehicle:
unchanged (no vehicle)
Details on exposure:
Each dose was administered over a 1-minute interval
Doses:
2.5, 5.0, and 10 g/kg
No. of animals per sex per dose:
5 males and 5 females
Details on study design:
- Duration of observation period following administration: 14 days
Statistics:
Median lethal dose (LD50), 95% confidence limit, probit slopes and maximum tolerated dose (LD0.1) were determined.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
5 750 mg/kg bw
Mortality:
With one exception, deaths occurred within the first 24 hours
Clinical signs:
Death was preceded by tremors, myasthenia, dyspnea, and occassionally, convulsions
Non-lethal doses of DMSO produced decreased motor activity and myasthenia

Any other information on results incl. tables

The LD0.1 was calculated to be 2.74 g/kg.

Applicant's summary and conclusion