Registration Dossier
Registration Dossier
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EC number: 203-646-3 | CAS number: 109-09-1
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 2-chloropyridine
- EC Number:
- 203-646-3
- EC Name:
- 2-chloropyridine
- Cas Number:
- 109-09-1
- Molecular formula:
- C5H4ClN
- IUPAC Name:
- 2-chloropyridine
- Details on test material:
- Batch Number: 9RC123498
Description: clear colourless liquid
Purity: 99.2%
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- Source: Harlan Laboratories UK Ltd
Acclimatisation period: 5 days
Weight at study initiation: 15-23g
Age at study intiation: 8-12 weeks
Temperature: 19-25 degrees C
Relative humidity: 30-70%
Photoperiod: 12:12
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 25% 10% 5% v/v
- No. of animals per dose:
- 4
- Details on study design:
- daily application for 3 consecutive days
25ul applied to dorsal surface of each ear
3H-methyl thymidine injected into tail vein on day 6 (250ul PBS containing 20uCi 3HTdR)
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: control: n/a 5%: 0.40 10%: 0.89 25%: 0.82
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: control: 16963.96 dpm 5%: 6847.05 dpm 10%: 15156.97 dpm 25%: 13912.59 dpm
Any other information on results incl. tables
No mortalities and no signs of systemic toxicity during test, body weight changes were comparable to those of the control animals.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- The test material was considered to be a non-sensitiser under the conditions of the test
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