2-chloropyridine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1964

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study without detailed documentation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1964

Report date:

1964

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Version / remarks:

reliability scoring based on 2001 guideline

Deviations:

yes

Remarks:

purity, source and phys/chem properties of test substance not reported, temperature, humidity, and light cycle not reported, and age of animals not reported

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Remarks:

study pre-dates GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloropyridine

EC Number:

203-646-3

EC Name:

2-chloropyridine

Cas Number:

109-09-1

Molecular formula:

C5H4ClN

IUPAC Name:

2-chloropyridine

Details on test material:

- Name of test material (as cited in study report): 2-chloropyridine
- Physical state: liquid
- Analytical purity: free of impurities
- Lot/batch No.: PD 646
- Expiration date of the lot/batch: Not reported
- Stability under test conditions: Not reported

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Not reported
- Age at study initiation: Not reported
- Weight at study initiation: 201 - 304 g
- Fasting period before study: 16 hours
- Housing: Individually in metal cages suspended above the droppings.
- Diet (e.g. ad libitum): food fed ad libitum
- Water (e.g. ad libitum): water provided ad libitum
- Acclimation period: Not reported


ENVIRONMENTAL CONDITIONS
- Temperature (°C): Not reported
- Humidity (%): Npt reported
- Air changes (per hr): Not reported

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: methycellulose

Details on oral exposure:

The compound was administered orally as a suspension in a 0.5 per cent aqueous dispersion of methycellulose. Each dose level was prepared in a concentration which enabled the delivery of a constant volume of 1.0 mL/100 g body weight.

Doses:

100, 215, 464, 681, 1000, 1470, or 2150 mg/kg body weight

No. of animals per sex per dose:

6 rats/dose

Control animals:

other: not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: the animals were observed for mortality and adverse clinical signs at 4 hours, 24 hours, and daily thereafter.
- Necropsy of survivors performed: yes

Statistics:

LD50 was calculated

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

342 mg/kg bw

Mortality:

At dose levels of 681, 1000, 1470, and 2150 mg/kg body weight, all animals died.

Clinical signs:

other: Clinical signs included nasal porphyrin, hypoactivity, sedation, ataxia, dyspenia, hypersensitive to touch, sedation, hypnosis, ptosis, salivation, and reduced pain response.

Gross pathology:

Included unilateral hydronephrosis, pale liver with friable accentuated lobules, congested lungs, and hemorrhages of the stomach, intestines, and urinary bladder.