2-chloropyridine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation.

Data source

Reference

Reference Type:

secondary source

Title:

Unnamed

Year:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-methylpyridine

EC Number:

203-636-9

EC Name:

3-methylpyridine

Cas Number:

108-99-6

IUPAC Name:

3-methylpyridine

Details on test material:

- Name of test material (as cited in study report): 3-Methylpyridine

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Melting point: -18.1ºC
- Boiling point: 143-144ºC
- Vapour pressure: 6.05 mm Hg at 25ºC
- Water solubility (under test conditions):
- Henry's law constant: 7.73 x 10E-6 atm-cu m/mol at 25ºC
- log Pow: 1.20
- pKa: 5.63 at 25ºC

Reference: HSDB® is provided quarterly by US National Library of Medicine and was last updated December 18, 2009

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The growth medium used in the test was prepared according to the OECD guideline. The medium was filter-sterilized with a 0.45 µm membrane filter.

Test solutions were prepared by adding an aliquot from each of five stock solutions (1, 3.2, 10, 32 and 100 g test substance/L) to the appropriate test flasks.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

- Strain: CCAP 278-4
- Source (laboratory, culture collection): CCAP, UK
- Method of cultivation: The algae were cultured at the testing facility for several weeks in Boltz Basal Medium (BBM).

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

25 ± 1ºC

pH:

Not reported in summary.

Nominal and measured concentrations:

Nominal concentrations: 0, 10, 32, 100, 320 and 1000 mg/L

Details on test conditions:

Test vessels were all-glass 100-mL Erlenmeyer flasks containing 50 mL of test solution. Triplicate flasks were used at each test concentration. Test solutions were prepared by adding an aliquot from each of five stock solutions (1, 3.2, 10, 32 and 100 g test substance/L) to the appropriate test flasks. Flasks were inoculated with algal cells to achieve an initial cell density of 10E4 cells/mL. Flasks were incubated n an environmental cabinet under continuous illumination at a temperature of 25 ± 1ºC. Flasks were continuously shaken during the experiment. Cell counts were made after 3 days of incubation using a Neubauer counting chamber. The average specific growth rate was calculated for each flask and the average growth rate for each treatment was compared to the control group. The pH of the test solutions was measured in each flask at the beginning and end of the test.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Reported statistics and error estimates:

No statistics applied to data.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified