Fatty acids, C16-18 and C18-unsatd., Me esters, epoxidized, reaction products with ethylene glycol

Endpoint summary

Additional information

Toxicity to fish:

The acute lethal toxicity of Fatty acids, C16 -18 and C18 -unsatd., Me esters, epoxidized, reaction products with ethylene glycol was investigated with an analogous substance fatty acids, C16 -18 and C18 -unsatd., Me esters, epoxidized, reaction products with trimethylolpropane (CAS 188831-96-1) under semi-static conditions with zebra-fish (Brachydanio rerio) over a period of 96 h according to the guideline ISO 7346/2. At loading rates between 4.27 mg/l and 100 mg/l, none of the fish (10 fish per concentration) died after 96h of exposure. Based on these results the LC₅₀is greater than the applied loading rate of 100 mg/l. (BASF, 1997)

The study was tested with a similar molecule, which has the same structural body, only the reaction products of the functional groups are different (instead of reaction products with ethylene glycol -> reaction products with trimethyolpropane). Both substances show similar structural, physico-chemical, environmental and toxicological properties.

Toxicity to daphnia:

The acute toxicity to invertebrates was determined according to OECD 202 with test substance preparations with 1, 3, 10, 30 and 100 mg/l since the test item is poor soluble in water. After 24h of stirring the test substance preparations were filtered. The results of this study showed that the test substance preparation of Fatty acids, C16 -18 and C18 -unsatd., Me esters, epoxidized, reaction products with ethylene glycol induced immobilsation to the exposed Daphnia magna within 48 hours. The EC₅₀ value after 2 days of exposure was 43 mg/l, based on the nominal concentrations.(BASF, 2009)

The chronic toxicity to invertebrates was determined according to OECD 211 as a limit test. The test substance is a liquid which is poorly soluble in water. Therefore the test solutions were prepared in order to generate a water accommodated fraction (WAF) of the test substance in test medium. No significant mortality or any other additional significant adverse effects or abnormal behavior were observed in any of the test treatments. Thus, the No Observed Effect (NOEC) concentration was > 10 mg/l. (BASF, 2013)

Toxicity to algae:

The purpose of this study according to OECD 201 was to determine the effect of the test item on the growth of the freshwater green alga, Desmodesmus subspicatus. The loading rates of Fatty acids, C16 -18 and C18 -unsatd., Me esters, epoxidized, reaction products withe ethylene glycol were 0.316, 1.00, 3.16, 10.0, 31.6 and 100 mg/l. Since Fatty acids, C16 -18 and C18 -unsatd., Me esters, epoxidized, reaction products withe ethylene glycol is a multi-component substance that is only partially soluble, the water accomodated fraction (WAF) was used.

The 72 -h ErC50 was estimated to be greater than 100 mg/L and the 72 -hour ErC10 was calculated to be 3.74 mg/L based on nominal loading rates (BASF, 2013)

Toxicity to microorganism:

The objective of this study according to OECD 209 was to determine the effect of different concentrations of the test item on sewage sludge microorganisms by measuring the respiration rate under defined conditions. The EC10 value for Fatty acids, C16 -18 and C18 -unsatd., Me esters, epoxidized, reaction products with ethylene glycol was calculated to be greater than 1000 mg/L. (BASF, 2012)

In addition, a supporting study is available for the toxicity of fatty acids, C16 -18 and C18 -unsatd., Me esters, epoxidized, reaction products with trimethylolpropane (CAS 188831-96-1) to aquatic microorganisms. The test item was tested in the oxygen consumption inhibition test according to DIN 38412, Teil 27. A bacterial suspension of Pseudomonas putida was exposed to a nominal concentration range of 30 to 10000 mg/L of the test item. The test item was directly weighed into the test vessel. The oxygen consumption inhibition was measured in relation to three blank samples. The maximum inhibition value of the test sample was less than 10 % in relation to the blank samples. The EC0 was determined to 10000 mg/L. The EC10 was estimated to be > 10000 mg/L (Henkel, 1996).