Butanenitrile, 2-amino-2,3-dimethyl-

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1989

Reference 2

Reference Type:

publication

Title:

Robust Summary for Aminoalkylnitrile Category

Author:

US EPA HPVIS

Year:

2006

Bibliographic source:

Doc ID 201-16274

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-2,3-dimethylbutanenitrile

Cas Number:

13893-53-3

Molecular formula:

C6H12N2

IUPAC Name:

2-amino-2,3-dimethylbutanenitrile

Details on test material:

Purity: 96% in toluene

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Housing: five per sex.
Acclimatisation: At least 5 days prior to exposure.
Rats were kept on a 12 hour photoperiod. Pelleted feed and water were available ad libitum, except during exposure.
Females were nulliparous and non-pregnant.
Age: from 51 to approximately 73-85 days (age of 4 rats is not definitely known.)

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Details on inhalation exposure:

The rats were exposed in a 900-liter (approximate volume) glass and steel chamber.
5 male/5 female animals per dose group were exposed once for 4 hours to a vapor atmosphere dynamically generated from AMN.
Liquid AMN was metered with a sage model 355 syringe pump in a heated glass evaporator. The resultant vapor was carried into the chamber by an inlet stream of nitrogen, that entered the top of the evaporator. The resultant vapour was diluted to the desired vapour concentration and dispersed throughout the chamber by filtered supply air.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

each exposure with GC-FID

Duration of exposure:

4 h

Concentrations:

77, 71, 58, 21 ppm (analytical)

No. of animals per sex per dose:

5 animals per sex per dose

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

73 ppm

95% CL:

67 - 79

Exp. duration:

4 h

Remarks on result:

other: Vapor

Effect levels 2

Sex:

male/female

Dose descriptor:

LC50

Effect level:

0.34 mg/L air

95% CL:

> 0.31 - < 0.37

Exp. duration:

4 h

Remarks on result:

other: Vapor

Effect levels 3

Sex:

male/female

Dose descriptor:

LC50

Effect level:

340 mg/m³ air

Exp. duration:

4 h

Remarks on result:

other: Vapor

Any other information on results incl. tables

All deaths occured during time of exposure.

Table 1: Overview over the mortalities in the various dose groups:

Group	Mortality		Conc. (ppm)
	Male	Female
4 hour (96%)	2/5	5/5	77
	3/5	1/5	71
	0/5	0/5	58
	0/5	0/5	21

Clinical signs

0.36 mg/l: hypoactivity, prostration during exposure period.

0.33 mg/l: hypoactivity, prostration, increased respiration rate, ataxia was observed during exposure period.

No macroscopic lesions.