Butanenitrile, 2-amino-2,3-dimethyl-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA-660-3-75-009

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-2,3-dimethylbutanenitrile

Cas Number:

13893-53-3

Molecular formula:

C6H12N2

IUPAC Name:

2-amino-2,3-dimethylbutanenitrile

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

- Test concentrations were prepared by preparing a stock solution in deionized water and serially diluting to obtain desired concentration.

Test organisms

Test organisms (species):

Lepomis macrochirus

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22 +/-1 °C

Nominal and measured concentrations:

- The preliminary test concentrations were set at 0.1, 1.0, and 10 mg/L.
- Based on the results of the preliminary testing, five test concentrations (nominal) were selected, 0.10, 0.18, 0.32, 0.56, and 1.0 mg/L.

Details on test conditions:

- The static fish bioassay was conducted in five gallon glass vessels containing 15 liters of soft reconstituted water.
- 10 fish with a mean weight of 0.34 g and a mean length of 25 mm were used for each test concentration.
- The test vessels were kept in a water bath at 22 (±1) °C.
- A 48-hour range-finding test was conducted to determine the concentration range for the definitive study.
- All results were based on the nominal concentrations.
- The bluegill sunfish (Lepomis macrochirus) were challenged with a reference compound, Antimycin A, to verify that the fish were in good condition. - The 96-hour LC50 for bluegill sunfish exposed to the control substance was 1.2 × 10-4 mg/L, which indicates that the fish were in good condition. - The fish were observed once every 24 hours for mortality and abnormal effects. The no-effect concentration for the test material, based on the lack of mortality and abnormal effects, was estimated to be 0.56 mg/L after 96 hours.
- All fish in the 1.0 mg/L test concentration died on or before the 24-hour observation period.
- Water quality parameters of temperature, dissolved oxygen and pH were measured throughout the test and were within acceptable limits.
- Statistical analysis of the concentration vs. effect data was obtained by employing a computerized LC50 program.
- This program calculated the LC50 statistic and its 95% C.L. using the binomial and the moving average tests, respectively.
- The method of calculation selected for use was that which gave the narrowest confidence limits for the LC50.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.75 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)