trans-(5RS,6SR)-6-amino-2,2-dimethyl-1,3-dioxepan-5-ol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Remarks:

Inhibition of the growth of Pseudomonas putida

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

Details on results and parameters of test conditions are not documented in the report. Thus, the values obtained in this study are considered as being valid with restriction.

Qualifier:

according to guideline

Guideline:

other: ISO/TC 147/ SC 5/WG 1 N 111

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Pseudomonas putida
- Name and location of sewage treatment plant where inoculum was collected: "Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH" (DSM no: 50026)
- Method of cultivation: The bacteria were precultured by washing a stock culture on agar plates not more than 7 days old.
- Pretreatment: The preculture was diluted to obtain a FAU (Formazan Absorbance Units) of 10 /2/. After 7 hours incubation at 20 °C the preculture was used as inoculum to give the test batches a FAU of 5.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test temperature:

20°C

Nominal and measured concentrations:

- Concentrations: 1, 10, 100, 1000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL conical flasks
- Negative Control: Yes
- Positive Control: Yes

OTHER TEST CONDITIONS
- Details on incubation: Batches were incubated on a shaker-table

EFFECT PARAMETERS MEASURED
- The turbidity was measured as absorbance at 436 mm at the start and the end of the incubation and the inhibition was calculated relative to the control batches.

TEST CONCENTRATIONS
- Test concentrations: 1, 10, 100, 1000 mg/L
- Spacing factor for test concentrations: 10

Reference substance (positive control):

yes

Remarks:

Pentachlorophenol (PCP) and Mercury chloride (HgCI2 )

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

16 h

Dose descriptor:

other: EC 20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Relevant effect levels:
EC50 was between 10-100 mg/L for PCP
EC50 was between 0.1-1 mg/L for HgCl2

Validity criteria for the measurement of the bacterial toxicity:

The study was conducted in accordance with ISO/TC 147/ SC 5/WG 1 N 111 "biological methods". The validation criteria stated in the guideline (a multiplication of 6-7 times in the control-batch) were fulfilled as the mean growth of the control batches was from FAU = 5 to FAU = 790, which is satisfying according to the validation criteria. However, details on results and parameters of test conditions are not documented in the report. Thus, the values obtained in this study are considered as being valid with restriction.

Validity criteria fulfilled:

yes

Remarks:

See 'Any other information on results incl. tables'.

Conclusions:

The test result shows no inhibition on the growth in concentration up to 1000 mg/L for the test substance (EC50 > 1000 mg/L).

Executive summary:

The study was performed to assess the toxicity of the test substance to bacteria. The study was conducted in accordance with the ISO/TC 147/ SC 5/WG 1 N 111 "biological methods". Test batches were Inoculated with Pseudomonas bacteria and exposed to the test substance at concentrations of 1, 10, 100 and 1000 mg/L (nominal concentration). The bacterial growth was estimated turbidometrically by measuring the absorbance at 436 nm before and after a 16 hours incubation period and the inhibition was calculated relative to the control batches. The test result shows no inhibition on the growth in concentration up to 1000 mg/L for the test substance (EC50 > 1000 mg/L).

Description of key information

The study was performed to assess the toxicity of the test substance to bacteria. The study was conducted in accordance with the ISO/TC 147/ SC 5/WG 1 N 111 "biological methods". Test batches were Inoculated with Pseudomonas bacteria and exposed to the test substance at concentrations of 1, 10, 100 and 1000 mg/L (nominal concentration). The bacterial growth was estimated turbidometrically by measuring the absorbance at 436 nm before and after a 16 hours incubation period and the inhibition was calculated relative to the control batches. The test result shows no inhibition on the growth in concentration up to 1000 mg/L for the test substance (EC50 > 1000 mg/L).