1H-Imidazolium, 1,3-diethyl-, acetate (1:1)

Endpoint summary

Description of key information

skin: not corrosive

eye: not irritating

Additional information

skin irritation:

An in vitro skin corrosion/irritation test was concucted with the test substance EEIM Acetat. The test consists of a topical exposure of the test substance to the surface of a human reconstituted epidermis model for 3 minutes and 1 hour followed by a cell viability test. Cell viability is measured by dehydrogenase conversion of the yellow, water-soluble MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), present in cell mitochondria, into a blue formazan salt that is measured quantitatively after isopropanol – extraction from the tissues. The optical density of the extracts of test substance treated tissues is compared to negative control values from tissue treated with highly de-ionized water and is expressed as relative tissue viability. Based on the observed results it was concluded, that EEIM Acetat does not show a corrosive potential in the EpiDerm ™ skin corrosion test under the test conditions chosen.

eye irritation:

The potential of EEIM Acetate to cause ocular irritation was assessed by a single topical application of 50 µL of the undiluted test substance to a reconstructed three dimensional human cornea model (EpiOcular™).
Two EpiOcular™ tissue samples were incubated with the test substance for 30 minutes followed by a 2-hours post-incubation period. Tissue destruction was determined by measuring the metabolic activity of the tissue after exposure/post-incubation using a colorimetric test. The reduction of mitochondrial dehydrogenase activity, measured by reduced formazan production after incubation with a tetrazolium salt (MTT) was chosen as endpoint. The formazan production of the test substance treated epidermal tissues is compared to that of negative control tissues. The quotient of the values indicates the relative tissue viability. The test substance is not able to reduce MTT directly. The mean viability of the test-substance treated tissues was 71%. Based on the observed results and applying the evaluation it was concluded, that EEIM Acetate does not show an eye irritation potential in the EpiOcular™ eye irritation test under the test conditions chosen.

The potential of EEIM Acetate to cause serious damage to the eyes was assessed by a single topical application of 750 µL of the undiluted test substance to the epithelial surface of isolated bovine corneas (BCOP).
Three corneas were treated with the test substance for 10 minutes followed by a 2-hours post-incubation period. Corneal opacity was measured quantitatively as the amount of light transmission through the cornea. Permeability was measured quantitatively as the amount of sodium fluorescein dye that passes across the full thickness of the cornea. Both measurements were used to calculate an In Vitro Irritancy Score of the test substance relative to the control corneas. Based on the observed results and applying the evaluation criteria it was concluded, that EEIM Acetate does not cause serious eye damage in the Bovine Corneal Opacity and Permeability Test (BCOP Test) under the test conditions chosen.

Justification for classification or non-classification

In an in vitro skin test, the substance was tested as non-corrosive. Since there are no data for irritating effect available, the substance is classified as skin irritant as an worst case scenario.

Based on the results of the skin and eye irritaing testing, the test item is classified as skin irritating cat. 2 (H315) according to Regulation (EC) No 1272/2008 (CLP).